A new way of biosensing using fluorescence energy transfer and Langmuir-Blodgett films

A method of detecting the binding of ligand molecules (mannosides) to a biological receptor (Con A) by Förster energy transfer from Langmuir-Blodgett (LB) films is presented. The LB films consist of pre-polymerized amphiphilic polymethacrylates containing a coumarin fluorophore (donor) and reactive...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 1993-03, Vol.11 (1), p.103-108
Main Authors: Siegmund, Hans-Ulrich, Becker, Arno
Format: Article
Language:English
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Summary:A method of detecting the binding of ligand molecules (mannosides) to a biological receptor (Con A) by Förster energy transfer from Langmuir-Blodgett (LB) films is presented. The LB films consist of pre-polymerized amphiphilic polymethacrylates containing a coumarin fluorophore (donor) and reactive groups to which biological receptors can be attached. Studies determining the stability of the system are described. The specific binding of rhodamine (acceptor)-labelled ligands and their competitive replacement by unlablled analytes is demonstrated. A simple dual-channel fluorescence detector optimized for this purpose is described as well.
ISSN:0925-4005
1873-3077
DOI:10.1016/0925-4005(93)85244-5