Histological Evidence for Dissociation of Lipid Peroxidation and Cell Necrosis in Bromotrichloromethane Hepatotoxicity in the Perfused Rat liver

Bromotrichloromethane (CBrCl3)-induced hepatic lipid peroxidation and cell necrosis were studied histologically and biochemically, using isolated perfused livers from phenobarbital-pretreated rats. Lipid peroxidation was assessed by fuchsin staining of the liver slices and release of thiobarbituric...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1991-01, Vol.56 (2), p.143-150
Main Authors: Masuda, Yasusuke, Yamamori, Yasuko
Format: Article
Language:English
Subjects:
Animals
Bromotrichloromethane - toxicity
Histocytochemistry
In Vitro Techniques
Lipid Peroxidation - drug effects
Liver - drug effects
Liver - metabolism
Liver - pathology
Male
Necrosis
Oxygen - administration & dosage
Oxygen - metabolism
Perfusion
Rats
Rats, Inbred Strains
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Summary:Bromotrichloromethane (CBrCl3)-induced hepatic lipid peroxidation and cell necrosis were studied histologically and biochemically, using isolated perfused livers from phenobarbital-pretreated rats. Lipid peroxidation was assessed by fuchsin staining of the liver slices and release of thiobarbituric acid reactive substances (TBARS) into the perfusate; necrosis was assessed by trypan blue uptake and lactate dehydrogenase (LDH) leakage. A good correlation was observed between the Schiff-positive reaction and TBARS release under various experimental conditions, supporting the validity of the fuchsin staining method for histological detection of lipid peroxidation. Lobular localization of lipid peroxidation and necrosis was as follows: Under high oxygen supply (95% O2-saturated buffer), infusion of CBrCl3 caused the Schiff-positive reaction in the pericentral to midzonal hepatocytes, irrespective of the direction of perfusion, but did not produce necrosis. Under low oxygen supply (20% O2) with retrograde perfusion, dissociation of lipid peroxidation and necrosis was observed, i.e., trypan blue uptake in the periportal zones and Schiff-positive staining in the pericentral hepatocytes. Thus, lipid peroxidation by itself may have a relatively minor role in the development of CBrCl3-induced acute hepatic cell death.
ISSN:0021-5198
1347-3506
DOI:10.1016/S0021-5198(19)39874-9