A piezoelectric immunobiosensor for atrazine in drinking water

A piezoelectric crystal immunobiosensor has been developed for the assay of atrazine herbicides in drinking water. Determinations from 0.3–100 μg 1 −1 (parts ber billion) of atrazine can be made with a relative SD of about ± 8%. Atrazine antibodies (polyclonal from sheep) are layered onto the gold e...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1992, Vol.7 (6), p.411-419
Main Authors: Guilbault, George G., Hock, Bertold, Schimid, Rolf
Format: Article
Language:English
Subjects:
analytical methods
antibodies
atrazine
Atrazine - analysis
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
crystals
detectors
drinking water
electrodes
Evaluation Studies as Topic
Fundamental and applied biological sciences. Psychology
herbicides
Herbicides - analysis
Immunoassay - methods
Immunoassay - statistics & numerical data
immunoassays
immunobiosensor
Methods. Procedures. Technologies
piezocrystal
piezocrystals
proteins
Sensitivity and Specificity
Various methods and equipments
Water Pollutants, Chemical - analysis
Water Supply - analysis
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Summary:A piezoelectric crystal immunobiosensor has been developed for the assay of atrazine herbicides in drinking water. Determinations from 0.3–100 μg 1 −1 (parts ber billion) of atrazine can be made with a relative SD of about ± 8%. Atrazine antibodies (polyclonal from sheep) are layered onto the gold electrode of 10 MHz piezoelectric crystals, which are precoated with protein A. The sensor is reversible, being reusable for about eight or nine assays.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(92)85040-H