Cloning and sequence analysis of the cDNA for human placental NAD super(+)-dependent 15-hydroxyprostaglandin dehydrogenase

NAD super(+)-dependent 15-hydroxyprostaglandin dehydrogenase catalyzes the oxidation of many prostaglandins at C-15, resulting in a subsequent reduction in their biological activity. We report the isolation of the cDNA for this enzyme. A 2.5-kilobase cDNA containing the entire coding region for the...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1990-01, Vol.265 (25), p.14888-14891
Main Authors: Ensor, C M, Yang, Ji-Yu, Okita, R T, Tai, Hsin-Hsiung
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:NAD super(+)-dependent 15-hydroxyprostaglandin dehydrogenase catalyzes the oxidation of many prostaglandins at C-15, resulting in a subsequent reduction in their biological activity. We report the isolation of the cDNA for this enzyme. A 2.5-kilobase cDNA containing the entire coding region for the enzyme was isolated. The cDNA encodes for a protein of 266 amino acids with a calculated M sub(r) of 28,975. Identification of the cDNA as that coding for 15-hydroxyprostaglandin dehydrogenase was based on the comparison of the deduced amino acid sequence with the amino acid sequence of two peptides, one from the rabbit lung enzyme and the other from the human placental enzyme. (DBO)
ISSN:0021-9258