mRNA m6A methylation downregulates adipogenesis in porcine adipocytes

Fat Mass and Obesity-associated protein (FTO), associated with obesity, is proved to demethylate N6-methyladenosine (m6A), which raises questions regarding whether m6A plays vital roles in adipogenesis. To prove this, overexpression and knockdown of FTO and METTL3, as well as the chemical treatment...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2015-04, Vol.459 (2), p.201-207
Main Authors: Wang, Xinxia, Zhu, Linna, Chen, Jingqing, Wang, Yizhen
Format: Article
Language:English
Subjects:
Adenosine - analogs & derivatives
Adenosine - chemistry
Adenosine - metabolism
Adipocytes - drug effects
Adipocytes - metabolism
Adipogenesis
Adipogenesis - drug effects
Adipogenesis - genetics
Adipogenesis - physiology
Animals
Betaine - pharmacology
Cells, Cultured
Cycloleucine - pharmacology
Down-Regulation
FTO
Gene Knockdown Techniques
Lipid Metabolism
m6A
Methylation
Methyltransferases - antagonists & inhibitors
Methyltransferases - genetics
Methyltransferases - metabolism
METTL3
Porcine adipocytes
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Messenger - metabolism
Swine
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Summary:Fat Mass and Obesity-associated protein (FTO), associated with obesity, is proved to demethylate N6-methyladenosine (m6A), which raises questions regarding whether m6A plays vital roles in adipogenesis. To prove this, overexpression and knockdown of FTO and METTL3, as well as the chemical treatment in procine adipocytes were conducted. The results showed FTO negatively regulated m6A levels and positively regulated adipogenesis, while METTL3 positively correlated with m6A levels and negatively with adipogenesis. To remove the potential effect of FTO and METTL3 gene, chemical reagents of methylation inhibitor cycloleucine and methyl donor betaine were used to test the regulation effect of m6A on adipogenesis. The results showed the inverse effect of m6A on lipid accumulation in porcine adipocytes. These findings provide compelling evidence that m6A plays a critical role in the regulation of adipogenesis. •FTO negatively regulated m6A levels and positively regulated adipogenesis.•METTL3 positively correlated with m6A levels and negatively with adipogenesis.•Cycloleucine negatively regulated m6A levels and positively regulated adipogenesis.•Betaine positively correlated with m6A levels and negatively with adipogenesis.•mRNA m6A methylation downregulates adipogenesis in porcine adipocytes.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2015.02.048