Enhanced Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Graphene Oxide-Incorporated Electrospun Poly(lactic-co-glycolic acid) Nanofibrous Mats

Currently, combining biomaterial scaffolds with living stem cells for tissue regeneration is a main approach for tissue engineering. Mesenchymal stem cells (MSCs) are promising candidates for musculoskeletal tissue repair through differentiating into specific tissues, such as bone, muscle, and carti...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2015-03, Vol.7 (11), p.6331-6339
Main Authors: Luo, Yu, Shen, He, Fang, Yongxiang, Cao, Yuhua, Huang, Jie, Zhang, Mengxin, Dai, Jianwu, Shi, Xiangyang, Zhang, Zhijun
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemical synthesis
Cell Differentiation - physiology
Cell Proliferation
Cells, Cultured
Electroplating - methods
Equipment Design
Equipment Failure Analysis
Female
Graphite - chemistry
Humans
Lactic Acid - chemistry
Materials Testing
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Nanofibers - chemistry
Nanofibers - ultrastructure
Osteoblasts - cytology
Osteoblasts - physiology
Osteogenesis - physiology
Oxides - chemistry
Particle Size
Polyglycolic Acid - chemistry
Pregnancy
Tissue Engineering - instrumentation
Tissue Engineering - methods
Tissue Scaffolds
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Summary:Currently, combining biomaterial scaffolds with living stem cells for tissue regeneration is a main approach for tissue engineering. Mesenchymal stem cells (MSCs) are promising candidates for musculoskeletal tissue repair through differentiating into specific tissues, such as bone, muscle, and cartilage. Thus, successfully directing the fate of MSCs through factors and inducers would improve regeneration efficiency. Here, we report the fabrication of graphene oxide (GO)-doped poly­(lactic-co-glycolic acid) (PLGA) nanofiber scaffolds via electrospinning technique for the enhancement of osteogenic differentiation of MSCs. GO-PLGA nanofibrous mats with three-dimensional porous structure and smooth surface can be readily produced via an electrospinning technique. GO plays two roles in the nanofibrous mats: first, it enhances the hydrophilic performance, and protein- and inducer-adsorption ability of the nanofibers. Second, the incorporated GO accelerates the human MSCs (hMSCs) adhesion and proliferation versus pure PLGA nanofiber and induces the osteogenic differentiation. The incorporating GO scaffold materials may find applications in tissue engineering and other fields.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.5b00862