Compartmentalization in penicillin G biosynthesis by Penicillium chrysogenum PQ-96

The arrangement of organelles in the sub-apical productive non-growing vacuolated hyphal cells of the high- and the low-penicillin-pro- ducing strains Penicillium chrysogenum was compared using transmission electron microscopy. In the productive cells of the high-yielding strain the endoplasmic reti...

Full description

Saved in:
Bibliographic Details
Published in:Polish journal of microbiology 2014, Vol.63 (4), p.399-408
Main Authors: Kurzątkowski, Wiesław, Staniszewska, Monika, Bondaryk, Małgorzata, Gębska-Kuczerowska, Anita
Format: Article
Language:English
Subjects:
Antibiotics
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological Transport
Biosynthesis
Biosynthetic Pathways
Fungi
Oxidoreductases - genetics
Oxidoreductases - metabolism
Penicillin
Penicillin G - metabolism
Penicillium chrysogenum - genetics
Penicillium chrysogenum - metabolism
Penicillium chrysogenum - ultrastructure
Phenylacetates - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The arrangement of organelles in the sub-apical productive non-growing vacuolated hyphal cells of the high- and the low-penicillin-pro- ducing strains Penicillium chrysogenum was compared using transmission electron microscopy. In the productive cells of the high-yielding strain the endoplasmic reticulum and the polyribosomes with associated peroxisomes are frequently arranged at the periphery of the cytoplasm and around the vacuoles. At the high activity of penicillin G biosynthesis the immuno-label of the cytosolic isopenicillin N synthase is concentrated at the polyribosomes arranged in the peripheral cytoplasm and along the tonoplast as well as around the peroxisomes. On the basis of the obtained results the compartmentalization of the pathway of penicillin G biosymthesis is discussed. The obtained results support the phenylacetic acid detoxification hypothesis of penicillin G biosynthesis.
ISSN:1733-1331
2544-4646
DOI:10.33073/pjm-2014-054