Platinated oligomers of bovine pancreatic ribonuclease: Structure and stability

The reaction between cis-diamminedichloroplatinum(II) (CDDP), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease (RNase A), induces extensive protein aggregation, leading to the formation of one dimer, one trimer and higher oligomers whose yields depend on cisplatin/protein rati...

Full description

Saved in:
Bibliographic Details
Published in:Journal of inorganic biochemistry 2015-05, Vol.146, p.37-43
Main Authors: Picone, Delia, Donnarumma, Federica, Ferraro, Giarita, Russo Krauss, Irene, Fagagnini, Andrea, Gotte, Giovanni, Merlino, Antonello
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cattle
Cisplatin
Cisplatin - chemistry
Enzyme Stability
Molecular Sequence Data
Platinated ribonucleases
Protein aggregation
Protein Binding
Protein Multimerization
Protein Subunits - chemistry
Protein Subunits - metabolism
Protein–metal interactions
Ribonuclease oligomers
Ribonuclease, Pancreatic - chemistry
Ribonuclease, Pancreatic - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The reaction between cis-diamminedichloroplatinum(II) (CDDP), cisplatin, a common anticancer drug, and bovine pancreatic ribonuclease (RNase A), induces extensive protein aggregation, leading to the formation of one dimer, one trimer and higher oligomers whose yields depend on cisplatin/protein ratio. Structural and functional properties of the purified platinated species, together with their spontaneous dissociation and thermally induced denaturation, have been characterized. Platinated species preserve a significant, although reduced, ribonuclease activity. The high resistance of the dimers against dissociation and the different thermal unfolding profiles suggest a quaternary structure different from those of the well-known swapped dimers of RNase A. RNase A incubation in the presence of an excess of cisplatin induces the formation of platinated oligomers with structural and functional properties distinct from those of the previously characterized oligomers of the same protein. [Display omitted] •Oligomeric forms of RNase A were obtained in the presence of cisplatin.•The yield of platinated RNase A oligomers depends on cisplatin/RNase A ratio.•Platinated RNase A derivatives were characterized by circular dichroism.•Platinated dimer properties differ from those of RNase A N- and C-swapped dimers.•Platinated monomer, dimer and trimer keep a significant ribonuclease activity.
ISSN:0162-0134
1873-3344
DOI:10.1016/j.jinorgbio.2015.02.011