Karyology and agar analysis of the agarophyte Gelidiella acerosa (Forsskål) Feldmann et Hamel from the Philippines

Microspectrophotometry with the DNA-localizing fluorochrome DAPI demonstrated ploidy level differences in tetrasporophytic and presumptive gametophytic phases of Gelidiella acerosa from the Philippines. Comparison of mean nuclear DNA (I sub(f)) values to chicken erythrocytes (RBC) resulted in an est...

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Bibliographic Details
Published in:Journal of applied phycology 1994-12, Vol.6 (5-6), p.545-550
Main Authors: KAPRAUN, D. F, GANZON-FORTES, E, BIRD, K. T, TRONO, G, BREDEN, C
Format: Article
Language:English
Subjects:
Algae
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gelidiella acerosa
Marine
Plant cytology, morphology, systematics, chorology and evolution
Thallophyta
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Summary:Microspectrophotometry with the DNA-localizing fluorochrome DAPI demonstrated ploidy level differences in tetrasporophytic and presumptive gametophytic phases of Gelidiella acerosa from the Philippines. Comparison of mean nuclear DNA (I sub(f)) values to chicken erythrocytes (RBC) resulted in an estimate of 0.32 pg/2 C genome. Karyological studies with aceto-orcein revealed a chromosome complement of six bivalents during diakinesis of tetrasporocytes. The agar yield ranges from 13-24% dry weight, depending on the method of extraction. Agar extraction in 1 N NaOH resulted in an increased gel strength of 189 g/cm super(2) at 1.5% concentration. Infrared spectroscopy indicated a relatively high sulfate content in native agar. The low (61 degree ) melting temperature is indicative of high sulfation and small molecular size.
ISSN:0921-8971
1573-5176
DOI:10.1007/BF02182409