Controlled expression of pectic enzymes in Arabidopsis thaliana enhances biomass conversion without adverse effects on growth

• Published in: Phytochemistry (Oxford) 2015-04, Vol.112, p.221-230
• Main Authors: Tomassetti, Susanna, Pontiggia, Daniela, Verrascina, Ilaria, Reca, Ida Barbara, Francocci, Fedra, Salvi, Gianni, Cervone, Felice, Ferrari, Simone
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis thaliana; Biofuel; Biomass; Cell wall; Gene Expression; Glucose - metabolism; Pectin; Pectobacterium - enzymology; Pectobacterium - genetics; Polysaccharide-Lyases - genetics; Polysaccharide-Lyases - metabolism; Saccharification
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2014.08.026

The controlled expression of pectic enzymes improves saccharification without effects on growth and development. [Display omitted] •Arabidopsis plants with inducible expression of a pectate lyase were generated.•Plants expressing a polygalacturonase during senescence (SAG12-PG) were generated.•Transgenic plants show increased saccharification when the transgene is expressed.•SAG12-PG plants have normal growth and development.•Controlled in planta expression of pectic enzymes can improve biofuel production. Lignocellulosic biomass from agriculture wastes is a potential source of biofuel, but its use is currently limited by the recalcitrance of the plant cell wall to enzymatic digestion. Modification of the wall structural components can be a viable strategy to overcome this bottleneck. We have previously shown that the expression of a fungal polygalacturonase (pga2 from Aspergillus niger) in Arabidopsis and tobacco plants reduces the levels of de-esterified homogalacturonan in the cell wall and significantly increases saccharification efficiency. However, plants expressing pga2 show stunted growth and reduced biomass production, likely as a consequence of an extensive loss of pectin integrity during the whole plant life cycle. We report here that the expression in Arabidopsis of another pectic enzyme, the pectate lyase 1 (PL1) of Pectobacterium carotovorum, under the control of a chemically inducible promoter, results, after induction of the transgene, in a saccharification efficiency similar to that of plants expressing pga2. However, lines with high levels of transgene induction show reduced growth even in the absence of the inducer. To overcome the problem of plant fitness, we have generated Arabidopsis plants that express pga2 under the control of the promoter of SAG12, a gene expressed only during senescence. These plants expressed pga2 only at late stages of development, and their growth was comparable to that of WT plants. Notably, leaves and stems of transgenic plants were more easily digested by cellulase, compared to WT plants, only during senescence. Expression of cell wall-degrading enzymes at the end of the plant life cycle may be therefore a useful strategy to engineer crops unimpaired in biomass yield but improved for bioconversion.