Baicalin can attenuate the inhibitory effects of mifepristone on Wnt pathway during peri-implantation period in mice

•Baicalin decreased E2, Dkk1 and increased P4,Wnt4, LRP6, β-catenin.•Mifepristone decreased Wnt4, LRP6, β-catenin and increased Dkk1.•Baicalin attenuate the inhibitory effects of mifepristone on Wnt pathway. Wnts are considered as important factors in uterus developmental process and embryo implanta...

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Published in:The Journal of steroid biochemistry and molecular biology 2015-05, Vol.149, p.11-16
Main Authors: Chen, J.G., Chen, T., Ding, Y., Han, L., Zhou, F.Y., Chen, W.Z., Ding, M.X.
Format: Article
Language:English
Subjects:
Abortifacient Agents, Steroidal - pharmacology
Animals
Baicalin
Embryo implantation
Embryo Implantation - drug effects
Estradiol - blood
Estradiol/progesterone
Female
Flavonoids - pharmacology
Gene Expression Regulation - drug effects
Mice
Mifepristone
Mifepristone - pharmacology
Pregnancy
Progesterone - blood
Tocolytic Agents - pharmacology
Wnt Signaling Pathway - drug effects
Wnt/β-catenin
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Summary:•Baicalin decreased E2, Dkk1 and increased P4,Wnt4, LRP6, β-catenin.•Mifepristone decreased Wnt4, LRP6, β-catenin and increased Dkk1.•Baicalin attenuate the inhibitory effects of mifepristone on Wnt pathway. Wnts are considered as important factors in uterus developmental process and embryo implantation. Baicalin has been demonstrated to possess tocolytic properties. In order to investigate the effect of baicalin on the Wnt signaling pathway during the peri-implantation, pregnant Kuming mice were randomly divided into four groups: control group, baicalin group administered with 40mg/kg BW of baicalin through an intragastric gavage on day 2 to 7 of the pregnancy (Pd2–Pd7), mifepristone group treated with 4mg/kg BW of mifepristone, an abortifacient agent, via subcutaneous administration on Pd4, and baicalin+mifepristone group treated with their combination. The concentrations of the implantation-related steroid hormones (progesterone and estradiol) in the blood serum were measured with RIA. The gene and protein expression levels of the important molecules of the Wnt pathway (Wnt4, LRP6, Dkk1 and β-catenin) in the endometrium were detected with RT-PCR and western blot, respectively. The results showed that baicalin decreased (P
ISSN:0960-0760
1879-1220
DOI:10.1016/j.jsbmb.2014.11.023