Synthesis of magnetic framework composites for the discrimination of Escherichia coli at the strain level

• Published in: Analytica chimica acta 2015-04, Vol.868, p.36-44
• Main Authors: Wei, Ji-Ping, Qiao, Bin, Song, Wen-Jun, Chen, Tao, li, Fei, Li, Bo-Zhi, Wang, Jin, Han, Ye, Huang, Yan-Feng, Zhou, Zhi-Jiang
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Proteins - chemistry; Biomarker; Biomarkers - chemistry; Cattle; Chemistry Techniques, Synthetic; Enrichment; Escherichia coli; Escherichia coli - chemistry; Escherichia coli - isolation & purification; Ferrosoferric Oxide - chemical synthesis; Ferrosoferric Oxide - chemistry; Magnetic framework composites; Magnets - chemistry; MALDI-TOF MS; Peptides - chemistry; Species Specificity
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2015.02.018

[Display omitted] •The Fe3O4-COOH@MIL-101 composites combined the excellent properties of MIL-101 and Fe3O4-COOH particles.•Fe3O4-COOH@MIL-101 composites exhibited the efficient protein enrichment properties, size-exclusion performance and magnetic responsiveness.•Fe3O4-COOH@MIL-101 composites were successfully applied in the selective enrichment of the protein biomarkers from bacterial cell lysates and the discrimination of Escherichia coli at the strain level. Rapid and efficient characterization and identification of pathogens at the strain level is of key importance for epidemiologic investigations, which still remains a challenge. In this work, solvothermically Fe3O4-COOH@MIL-101 composites were fabricated by in situ crystallization approach. The composites combine the excellent properties of both chromium (III) terephthalate (MIL-101) and carboxylic-functionalized magnetite (Fe3O4-COOH) particles and possess the efficient peptides/proteins enrichment properties and magnetic responsiveness. Fe3O4-COOH@MIL-101 composites as magnetic solid phase extraction materials were used to increase the discriminatory power of MALDI-TOF MS profiles. BSA tryptic peptides at a low concentration of 0.25fmolμL−1 could be detected by MALDI-TOF MS. In addition, Fe3O4-COOH@MIL-101 composites were successfully applied in the selective enrichment of the protein biomarkers from bacterial cell lysates and discrimination of Escherichia coli at the strain level. This work provides the possibility for wide applications of magnetic MOFs to discriminate pathogens below the species level.