Reagentless chemically modified carbon paste electrode based on a phenothiazine polymer derivative and yeast alcohol dehydrogenase for the analysis of ethanol

Amperometric biosensors for ethanol were constructed by immobilizing yeast alcohol dehydrogenase (ADH) on carbon paste (graphite powder: paraffin oil) chemically modified with a polymer to which a necessary mediator for electrocatalytic NADH oxidation had been covalently attached. Three different ap...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1993, Vol.8 (3), p.229-237
Main Authors: Domínguez, E., Lan, H.L., Okamoto, Y., Hale, P.D., Skotheim, T.A., Gorton, L., Hahn-Hägerdal, B.
Format: Article
Language:English
Subjects:
chemically modified carbon paste electrode
dehydrogenase
enzyme electrode
NAD +/NADH
polymer bound mediator
reagentless biosensor
Saccharomyces cerevisiae
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Summary:Amperometric biosensors for ethanol were constructed by immobilizing yeast alcohol dehydrogenase (ADH) on carbon paste (graphite powder: paraffin oil) chemically modified with a polymer to which a necessary mediator for electrocatalytic NADH oxidation had been covalently attached. Three different approaches were tested: adsorption of ADH on the electrode surface and adding NAD + to the contacting buffer; having both ADH and NAD + mixed into the paste; and co-immobilizing ADH and NAD + in the paste by adding polyethylenimine to the reaction mixture. The last approach resulted in the highest and fastest response. When inserted into a flow injection system, the biosensor responded linearly to ethanol between 2 μ m and 3 m m. The biosensor operates at +100 mV vs. Ag/AgCl.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(93)85038-P