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Reagentless chemically modified carbon paste electrode based on a phenothiazine polymer derivative and yeast alcohol dehydrogenase for the analysis of ethanol
Amperometric biosensors for ethanol were constructed by immobilizing yeast alcohol dehydrogenase (ADH) on carbon paste (graphite powder: paraffin oil) chemically modified with a polymer to which a necessary mediator for electrocatalytic NADH oxidation had been covalently attached. Three different ap...
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| Published in: | Biosensors & bioelectronics 1993, Vol.8 (3), p.229-237 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c437t-94701e5d7107b4170556550e5bc3bddafa15777d314d42ff0874c8b2d8f1bbd23 |
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| cites | cdi_FETCH-LOGICAL-c437t-94701e5d7107b4170556550e5bc3bddafa15777d314d42ff0874c8b2d8f1bbd23 |
| container_end_page | 237 |
| container_issue | 3 |
| container_start_page | 229 |
| container_title | Biosensors & bioelectronics |
| container_volume | 8 |
| creator | Domínguez, E. Lan, H.L. Okamoto, Y. Hale, P.D. Skotheim, T.A. Gorton, L. Hahn-Hägerdal, B. |
| description | Amperometric biosensors for ethanol were constructed by immobilizing yeast alcohol dehydrogenase (ADH) on carbon paste (graphite powder: paraffin oil) chemically modified with a polymer to which a necessary mediator for electrocatalytic NADH oxidation had been covalently attached. Three different approaches were tested: adsorption of ADH on the electrode surface and adding NAD
+ to the contacting buffer; having both ADH and NAD
+ mixed into the paste; and co-immobilizing ADH and NAD
+ in the paste by adding polyethylenimine to the reaction mixture. The last approach resulted in the highest and fastest response. When inserted into a flow injection system, the biosensor responded linearly to ethanol between 2 μ
m and 3 m
m. The biosensor operates at +100 mV vs. Ag/AgCl. |
| doi_str_mv | 10.1016/0956-5663(93)85038-P |
| format | article |
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+ to the contacting buffer; having both ADH and NAD
+ mixed into the paste; and co-immobilizing ADH and NAD
+ in the paste by adding polyethylenimine to the reaction mixture. The last approach resulted in the highest and fastest response. When inserted into a flow injection system, the biosensor responded linearly to ethanol between 2 μ
m and 3 m
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+ to the contacting buffer; having both ADH and NAD
+ mixed into the paste; and co-immobilizing ADH and NAD
+ in the paste by adding polyethylenimine to the reaction mixture. The last approach resulted in the highest and fastest response. When inserted into a flow injection system, the biosensor responded linearly to ethanol between 2 μ
m and 3 m
m. The biosensor operates at +100 mV vs. Ag/AgCl.</description><subject>chemically modified carbon paste electrode</subject><subject>dehydrogenase</subject><subject>enzyme electrode</subject><subject>NAD +/NADH</subject><subject>polymer bound mediator</subject><subject>reagentless biosensor</subject><subject>Saccharomyces cerevisiae</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNp9kU-L1TAUxYMo-JzxG7jISnRRTV6apt0IMvgPBmaQcR1ukxsbSZuaZB50Poyf1dQnLl3dxTn3d7nnEPKCszec8e4tG2TXyK4TrwbxupdM9M3tI3LgvRJNexTyMTn8szwlz3L-wRhTfGAH8usrwndcSsCcqZlw9gZC2OgcrXceLTWQxrjQFXJBigFNSdEiHSFXsQpA1wmXWCYPD35BusawzZioxeRPUPwJKSyWblgBFIKJUwxVnDabYj1cMdTFRMu0-yBs2WcaHcUywRLDJXniIGR8_ndekG8fP9xdfW6ubz59uXp_3ZhWqNIMrWIcpVWcqbHliknZSclQjkaM1oIDLpVSVvDWtkfnWK9a049H2zs-jvYoLsjLM3dN8ec95qJnnw2GAAvG-6x51ynZid3Yno0mxZwTOr0mP0PaNGd6L0PvSes9aT0I_acMfVvX3p3XsD5x8ph0Nh4Xg9anGqm20f8f8Bs7T5Vz</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Domínguez, E.</creator><creator>Lan, H.L.</creator><creator>Okamoto, Y.</creator><creator>Hale, P.D.</creator><creator>Skotheim, T.A.</creator><creator>Gorton, L.</creator><creator>Hahn-Hägerdal, B.</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>1993</creationdate><title>Reagentless chemically modified carbon paste electrode based on a phenothiazine polymer derivative and yeast alcohol dehydrogenase for the analysis of ethanol</title><author>Domínguez, E. ; Lan, H.L. ; Okamoto, Y. ; Hale, P.D. ; Skotheim, T.A. ; Gorton, L. ; Hahn-Hägerdal, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-94701e5d7107b4170556550e5bc3bddafa15777d314d42ff0874c8b2d8f1bbd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>chemically modified carbon paste electrode</topic><topic>dehydrogenase</topic><topic>enzyme electrode</topic><topic>NAD +/NADH</topic><topic>polymer bound mediator</topic><topic>reagentless biosensor</topic><topic>Saccharomyces cerevisiae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Domínguez, E.</creatorcontrib><creatorcontrib>Lan, H.L.</creatorcontrib><creatorcontrib>Okamoto, Y.</creatorcontrib><creatorcontrib>Hale, P.D.</creatorcontrib><creatorcontrib>Skotheim, T.A.</creatorcontrib><creatorcontrib>Gorton, L.</creatorcontrib><creatorcontrib>Hahn-Hägerdal, B.</creatorcontrib><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Domínguez, E.</au><au>Lan, H.L.</au><au>Okamoto, Y.</au><au>Hale, P.D.</au><au>Skotheim, T.A.</au><au>Gorton, L.</au><au>Hahn-Hägerdal, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reagentless chemically modified carbon paste electrode based on a phenothiazine polymer derivative and yeast alcohol dehydrogenase for the analysis of ethanol</atitle><jtitle>Biosensors & bioelectronics</jtitle><date>1993</date><risdate>1993</risdate><volume>8</volume><issue>3</issue><spage>229</spage><epage>237</epage><pages>229-237</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Amperometric biosensors for ethanol were constructed by immobilizing yeast alcohol dehydrogenase (ADH) on carbon paste (graphite powder: paraffin oil) chemically modified with a polymer to which a necessary mediator for electrocatalytic NADH oxidation had been covalently attached. Three different approaches were tested: adsorption of ADH on the electrode surface and adding NAD
+ to the contacting buffer; having both ADH and NAD
+ mixed into the paste; and co-immobilizing ADH and NAD
+ in the paste by adding polyethylenimine to the reaction mixture. The last approach resulted in the highest and fastest response. When inserted into a flow injection system, the biosensor responded linearly to ethanol between 2 μ
m and 3 m
m. The biosensor operates at +100 mV vs. Ag/AgCl.</abstract><pub>Elsevier B.V</pub><doi>10.1016/0956-5663(93)85038-P</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0956-5663 |
| ispartof | Biosensors & bioelectronics, 1993, Vol.8 (3), p.229-237 |
| issn | 0956-5663 1873-4235 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16675632 |
| source | ScienceDirect Physical & Analytical Chemistry Backfile |
| subjects | chemically modified carbon paste electrode dehydrogenase enzyme electrode NAD +/NADH polymer bound mediator reagentless biosensor Saccharomyces cerevisiae |
| title | Reagentless chemically modified carbon paste electrode based on a phenothiazine polymer derivative and yeast alcohol dehydrogenase for the analysis of ethanol |
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