β-Amyloid Precursor Protein Binds to the Neurite-Promoting IKVAV Site of Laminin

We previously characterized a 110-kDa membrane-associated laminin-binding protein (LBP110) from brain which binds the laminin A chain -Ile-Lys-Val-Ala-Val-(IKVAV) site and increases in injury. Here we demonstrate that antisera directed against different epitopes of β-amyloid precursor protein (APP)...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-11, Vol.90 (21), p.10150-10153
Main Authors: Kibbey, Maura C., Jucker, Mathias, Weeks, Benjamin S., Neve, Rachael L., Van Nostrand, William E., Kleinman, Hynda K.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amyloid beta-Protein Precursor - biosynthesis
Amyloid beta-Protein Precursor - metabolism
Animals
Antibodies
Binding Sites
Brain
Cell lines
Cells
Epitopes
Genetic Vectors
Laminin - chemistry
Laminin - metabolism
Molecular Sequence Data
Nerve Growth Factors - pharmacology
Neurites
Neurites - physiology
Neurons
Neuroscience
PC12 Cells
Peptide Fragments - chemistry
Peptide Fragments - metabolism
Proteins
RNA, Antisense
Transfection
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We previously characterized a 110-kDa membrane-associated laminin-binding protein (LBP110) from brain which binds the laminin A chain -Ile-Lys-Val-Ala-Val-(IKVAV) site and increases in injury. Here we demonstrate that antisera directed against different epitopes of β-amyloid precursor protein (APP) recognize LBP110 and that APP is recognized by LBP110 antiserum. APP specifically binds IKVAV and not another biologically active laminin-derived peptide containing the amino acid sequence -Tyr-Ile-Gly-Ser-Arg-. PC-12 cells transfected with antisense APP RNA produce less APP and LBP110, and they form fewer processes when cultured on either laminin or the IKVAV peptide. Thus, LBP110 is a member of the APP family and a function for APP in neurite outgrowth is now defined.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.90.21.10150