Characterization of a new ViI-like Erwinia amylovora bacteriophage phiEa2809

Erwinia amylovora is a Gram-negative plant pathogenic bacteria causing fire blight disease in many Rosaceae species.A novel E. amylovora bacteriophage, phiEa2809, was isolated from symptomless apple leaf sample collected in Belarus. This phage was also able to infect Pantoea agglomerans strains. The...

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Bibliographic Details
Published in:FEMS microbiology letters 2015-04, Vol.362 (7)
Main Authors: Lagonenko, Alexander L., Sadovskaya, Olga, Valentovich, Leonid N., Evtushenkov, Anatoly N.
Format: Article
Language:English
Subjects:
Blight
Contractility
Deoxyribonucleic acid
DNA
Erwinia amylovora
Erwinia amylovora - virology
Genome, Viral
Genomes
Icosahedral phase
Microbiology
Morphology
Myoviridae - classification
Myoviridae - genetics
Myoviridae - isolation & purification
Myoviridae - ultrastructure
Open Reading Frames
Pantoea - virology
Phages
Plant bacterial diseases
Plant Diseases - microbiology
Salmonella
Sequence Alignment
Sequence Analysis, DNA
tRNA
Virion
Virions
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Summary:Erwinia amylovora is a Gram-negative plant pathogenic bacteria causing fire blight disease in many Rosaceae species.A novel E. amylovora bacteriophage, phiEa2809, was isolated from symptomless apple leaf sample collected in Belarus. This phage was also able to infect Pantoea agglomerans strains. The genome of phiEa2809 is a double-stranded linear DNA 162 160 bp in length, including 145 ORFs and one tRNA gene. The phiEa2809 genomic sequence is similar to the genomes of the Serratia plymutica phage MAM1, Shigella phage AG-3, Dickeya phage vB DsoM LIMEstone1 and Salmonella phage ViI and lacks similarity to described E. amylovora phage genomes. Based on virion morphology (an icosahedral head, long contractile tail) and genome structure, phiEa2809 was classified as a member of Myoviridae, ViI-like bacteriophages group. PhiEa2809 is the firstly characterized ViI-like bacteriophage able to lyse E. amylovora. In this study, we investigated morphologic and genomic features of the new Erwinia amylovora bacteriophage phiEa2809 isolated from plant sample collected in Belarus.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnv031