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Pasteurella haemolytica leukotoxin-induced synthesis of eicosanoids by bovine neutrophils in vitro
Exposure of isolated bovine neutrophils to partially purified Pasteurella haemolytica leukotoxin caused increased synthesis of leukotriene B4 (LTB4) but not thromboxane B2 (TXB2) from endogenous arachidonic acid. Synthesis of LTB4 was closely correlated with leukotoxin‐induced neutrophil lysis. At l...
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Published in: | Journal of leukocyte biology 1994-11, Vol.56 (5), p.644-649 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Exposure of isolated bovine neutrophils to partially purified Pasteurella haemolytica leukotoxin caused increased synthesis of leukotriene B4 (LTB4) but not thromboxane B2 (TXB2) from endogenous arachidonic acid. Synthesis of LTB4 was closely correlated with leukotoxin‐induced neutrophil lysis. At low toxin concentrations, LTB4 production lagged behind leukotoxin‐induced neutrophil lysis over a 3‐h period. The neutralizing monoclonal antileukotoxin antibody MM601 neutralized both leukotoxin‐induced neutrophil lysis and LTB4 synthesis. Both leukotoxin‐induced neutrophil lysis and LTB4 synthesis were Ca2+‐devendent. When leukotoxin‐induced LTB4 synthesis from exogenous arachidonic acid was examined, significant LTB4 synthesis occurred at 5 min of leukotoxin exposure, which was before leukotoxin‐induced lysis developed. Leukotoxin‐induced LTB4 synthesis from endogenous arachidonic acid appears to require leukotoxin‐induced plasma membrane damage (occurring during neutrophil lysis), whereas LTB4 synthesis from exogenous arachidonic acid is initiated rapidly and occurs in the absence of plasma membrane damage. J. Leukoc. Biol. 56: 644–649; 1994. |
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ISSN: | 0741-5400 1938-3673 |
DOI: | 10.1002/jlb.56.5.644 |