Phosphatase activity and culture conditions of the yeast Candida mycoderma sp. and analysis of organic phosphorus hydrolysis ability

• Published in: Journal of environmental sciences (China) 2014-11, Vol.26 (11), p.2315-2321
• Main Authors: Yan, Mang, Yu, Liufang, Zhang, Liang, Guo, Yuexia, Dai, Kewei, Chen, Yuru
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Available phosphorus; Biomass; Candida - enzymology; Candida - metabolism; Candida mycoderma; Culture Media; Hydrogen-Ion Concentration; Hydrolysis; Lecithin; Organic phosphorus compounds; Phosphoric Monoester Hydrolases - metabolism; Phosphorus - metabolism
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/j.jes.2014.09.008

Orthophosphate is an essential but limiting macronutrient for plant growth. About 67% cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form of organic phosphorus, which is unavailable for root uptake unless hydrolyzed by secretory acid phosphatases. Thus, many microorganisms with the ability to produce phosphatase have been exploited. In this work, the activity of an extracellular acid phosphatase and yeast biomass from Candida mycoderma was measured under different culture conditions, such as pH, temperature, and carbon source. A maximal phosphatase activity of 8.47×105±0.11×105U/g was achieved by C. Mycoderma in 36hr under the optimal conditions. The extracellular acid phosphatase has high activity over a wide pH tolerance range from 2.5 to 5.0 (optimum pH3.5). The effects of different phosphorus compounds on the acid phosphatase production were also studied. The presence of phytin, lecithin or calcium phosphate reduced the phosphatase activity and biomass yield significantly. In addition, the pH of the culture medium was reduced significantly by lecithin. The efficiency of the strain in releasing orthophosphate from organic phosphorus was studied in red soil (used in planting trees) and rice soil (originating as red soil). The available phosphorus content was increased by 230% after inoculating 20days in rice soil and decreased by 50% after inoculating 10days in red soil. This work indicates that the yeast strain C. mycoderma has potential application for enhancing phosphorus utilization in plants that grow in rice soil.