Expression profile analysis of microRNAs in prostate cancer by next-generation sequencing

BACKGROUND Prostate cancer (PCa) is the second leading cause of tumor mortality among males in western societies. In China, the diagnostic and fatality rate of PCa is increasing yearly. METHODS To characterize underlying molecular mechanisms, the microRNA (miRNA) profile of high‐grade PCa, low‐grade...

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Bibliographic Details
Published in:The Prostate 2015-04, Vol.75 (5), p.500-516
Main Authors: Song, Chunjiao, Chen, Huan, Wang, Tingzhang, Zhang, Weiguang, Ru, Guomei, Lang, Juan
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
China
Computational Biology
Gene Expression Profiling
Genes
High-Throughput Nucleotide Sequencing
Humans
Male
Medical research
microRNA
MicroRNAs - genetics
Middle Aged
next-generation sequencing
pathway enrichment analysis
Prostate cancer
Prostatic Neoplasms - genetics
Real-Time Polymerase Chain Reaction
Rodents
Signal transduction
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Summary:BACKGROUND Prostate cancer (PCa) is the second leading cause of tumor mortality among males in western societies. In China, the diagnostic and fatality rate of PCa is increasing yearly. METHODS To characterize underlying molecular mechanisms, the microRNA (miRNA) profile of high‐grade PCa, low‐grade PCa, and benign prostate hyperplasia (BPH) were compared using high‐throughput Illumina sequencing and quantitative real‐time PCR (qRT‐PCR) methods. Moreover, a variety of biological information softwares and databases were applied to predict the target genes of miRNA, molecular functions, and signal pathways. RESULTS Eighteen miRNAs were differentially expressed (fold change ≥2, P 
ISSN:0270-4137
1097-0045
DOI:10.1002/pros.22936