Regulation of the microRNA processor DGCR8 by hepatitis B virus proteins via the transcription factor YY1

MicroRNAs (miRNAs) are a new class of well-conserved small noncoding RNAs that mediate posttranscriptional gene regulation. Hepatitis B virus (HBV) causes various liver diseases, including chronic hepatitis, liver cirrhosis and hepatocellular cancer. Recent data have indicated HBV alters miRNAs expr...

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Bibliographic Details
Published in:Archives of virology 2015-03, Vol.160 (3), p.795-803
Main Authors: Shan, Xuefeng, Ren, Min, Chen, Ke, Huang, Ailong, Tang, Hua
Format: Article
Language:English
Subjects:
Antigens
Biomedical and Life Sciences
Biomedicine
Brief Report
Cell Line
Enzymes
Gene Expression Regulation
Hepatitis B
Hepatitis B virus
Hepatitis B virus - physiology
Hepatocytes - virology
Host-Pathogen Interactions
Humans
Infections
Infectious Diseases
Liver cancer
Liver cirrhosis
Liver diseases
Medical Microbiology
MicroRNAs
MicroRNAs - metabolism
Proteins
RNA polymerase
RNA-Binding Proteins - metabolism
Signal transduction
Transcription factors
Viral Proteins - metabolism
Virology
Viruses
YY1 Transcription Factor - metabolism
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Summary:MicroRNAs (miRNAs) are a new class of well-conserved small noncoding RNAs that mediate posttranscriptional gene regulation. Hepatitis B virus (HBV) causes various liver diseases, including chronic hepatitis, liver cirrhosis and hepatocellular cancer. Recent data have indicated HBV alters miRNAs expression patterns, but the underlying mechanisms have not been fully established so far. Here, we provide a hypothesis that HBV alters the expressions of miRNAs by playing a role in the microRNA production process. In this study, we demonstrate that HBV downregulates miRNAs processor DGCR8 mRNA and protein expression in stable and transient HBV-expressing cells. HBV downregulates DGCR8 expression by inhibiting its promoter activity, and HBs and HBx may be involved in this process. Ectopic expression and knockdown of YY1 revealed that YY1 suppresses the activity of the DGCR8 promoter, while YY1 expression is significantly upregulated by HBV. In conclusion, our data show that HBV proteins repress DGCR8 promoter activity by upregulating the expression of transcription factor YY1. This provides a new insight into the mechanism of HBV-induced miRNA dysregulation.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-014-2286-x