Isolation and identification of efficient Egyptian malathion-degrading bacterial isolates

Bacterial isolates degrading malathion were isolated from the soil and agricultural waste water due to their ability to grow on minimal salt media amended with malathion as a sole carbon source. Efficiencies of native Egyptian bacterial malathion‐degrading isolates were investigated and the study ge...

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Bibliographic Details
Published in:Journal of basic microbiology 2015-03, Vol.55 (3), p.331-337
Main Authors: Hamouda, S. A., Marzouk, M. A., Abbassy, M. A., Abd-El-Haleem, D. A., Shamseldin, Abdelaal
Format: Article
Language:English
Subjects:
Acinetobacter
Acinetobacter - genetics
Bacillus
Bacillus - genetics
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
Biodegradation, Environmental
Bioremediation
Chromatography, Gas
DNA, Ribosomal - genetics
Egypt
Malathion
Malathion - metabolism
Molecular identification
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Soil Microbiology
Waste Water - microbiology
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Summary:Bacterial isolates degrading malathion were isolated from the soil and agricultural waste water due to their ability to grow on minimal salt media amended with malathion as a sole carbon source. Efficiencies of native Egyptian bacterial malathion‐degrading isolates were investigated and the study generated nine highly effective malathion‐degrading bacterial strains among 40. Strains were identified by partial sequencing of 16S rDNA analysis. Comparative analysis of 16S rDNA sequences revealed that these bacteria are similar with the genus Acinetobacter and Bacillus spp. and RFLP based PCR of 16S rDNA gave four different RFLP patterns among strains with enzyme HinfI while with enzyme HaeI they gave two RFLP profiles. The degradation rate of malathion in liquid culture was estimated using gas chromatography. Bacterial strains could degrade more than 90% of the initial malathion concentration (1000 ppm) within 4 days.
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201300220