Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg)

In this study, a cytochemical method and transmission electron microscopy was used to examine acid phosphatase activities of yolk granules throughout the early developmental stages of the Pacific oyster Crassostrea gigas. This study aimed to investigate the dynamic change of yolk granule acid phosph...

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Bibliographic Details
Published in:Chinese journal of oceanology and limnology 2015-03, Vol.33 (2), p.339-346
Main Authors: Wang, Yiyan, Sun, Hushan, Wang, Yanjie, Yan, Dongchun, Wang, Lei
Format: Article
Language:English
Subjects:
Acid phosphatase
Biology
Blastula
Crassostrea gigas
Degradation
Developmental stages
early development
Earth and Environmental Science
Earth Sciences
Egg yolk
Electron density
Electron microscopy
Embryogenesis
Embryonic growth stage
Enzymes
Granular materials
Larvae
Larval development
Larval stage
Marine molluscs
Microorganisms
Mollusca
Mollusks
morula
Oceanography
Oysters
Phosphatase
Transmission
Transmission electron microscopy
Veligers
Yolk
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Summary:In this study, a cytochemical method and transmission electron microscopy was used to examine acid phosphatase activities of yolk granules throughout the early developmental stages of the Pacific oyster Crassostrea gigas. This study aimed to investigate the dynamic change of yolk granule acid phosphatase, and the mechanisms underlying its involvement in yolk degradation during the early developmental stages of molluscs. Three types of yolk granules (YGI, YGII, and YGIII) that differed in electron density and acid phosphatase reaction were identified in early cleavage, morula, blastula, gastrula, trochophore, and veliger stages. The morphological heterogeneities of the yolk granules were related to acid phosphatase activity and degrees of yolk degradation, indicating the association of acid phosphatase with yolk degradation in embryos and larvae of molluscs. Fusion of yolk granules was observed during embryogenesis and larval development of C. gigas. The fusion of YGI (free of acid phosphatase reaction) with YGII (rich in acid phosphatase reaction) could be the way by which yolk degradation is triggered.
ISSN:0254-4059
2096-5508
1993-5005
2523-3521
DOI:10.1007/s00343-015-3297-5