Melatonin induces class A1 heat-shock factors (HSFA1s) and their possible involvement of thermotolerance in Arabidopsis

Melatonin (N‐acetyl‐5‐methoxytryptamine) serves as an important signal molecule during plant developmental processes and multiple abiotic stress responses. However, the involvement of melatonin in thermotolerance and the underlying molecular mechanism in Arabidopsis were largely unknown. In this stu...

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Bibliographic Details
Published in:Journal of pineal research 2015-04, Vol.58 (3), p.335-342
Main Authors: Shi, Haitao, Tan, Dun-Xian, Reiter, Russel J., Ye, Tiantian, Yang, Fan, Chan, Zhulong
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - drug effects
Arabidopsis - physiology
Arabidopsis Proteins - analysis
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
class A1 heat-shock factors (HSFA1s)
heat-responsive genes
Heat-Shock Proteins - analysis
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
Heat-Shock Response - drug effects
melatonin
Melatonin - pharmacology
thermotolerance
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Summary:Melatonin (N‐acetyl‐5‐methoxytryptamine) serves as an important signal molecule during plant developmental processes and multiple abiotic stress responses. However, the involvement of melatonin in thermotolerance and the underlying molecular mechanism in Arabidopsis were largely unknown. In this study, we found that the endogenous melatonin level in Arabidopsis leaves was significantly induced by heat stress treatment, and exogenous melatonin treatment conferred improved thermotolerance in Arabidopsis. The transcript levels of class A1 heat‐shock factors (HSFA1s), which serve as the master regulators of heat stress responses, were significantly upregulated by heat stress and exogenous melatonin treatment in Arabidopsis. Notably, exogenous melatonin‐enhanced thermotolerance was largely alleviated in HSFA1s quadruple knockout (QK) mutants, and HSFA1s‐activated transcripts of heat‐responsive genes (HSFA2, heat stress‐associated 32 (HSA32), heat‐shock protein 90 (HSP90), and 101 (HSP101)) might be contributed to melatonin‐mediated thermotolerance. Taken together, this study provided direct link between melatonin and thermotolerance and indicated the involvement of HSFA1s‐activated heat‐responsive genes in melatonin‐mediated thermotolerance in Arabidopsis.
ISSN:0742-3098
1600-079X
DOI:10.1111/jpi.12219