Prognostic value of COX-2, P53, and EZH-2 evaluated by quantitative image analysis in premalignant and malignant breast lesions

• Published in: Diagnostic cytopathology 2015-04, Vol.43 (4), p.294-300
• Main Authors: Nicolopoulou-Stamati, Polyxeni, Tsipis, Angelos, Chelidonis, George, Patsouris, Efstratios, Athanassiadou, Pauline, Gonidi, Maria, Athanassiadou, Anna Maria
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor - metabolism; Biopsy, Fine-Needle; Breast - pathology; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; COX-2; Cyclooxygenase 2 - metabolism; Diagnosis, Differential; Enhancer of Zeste Homolog 2 Protein; EZH-2; Female; Humans; Hyperplasia - metabolism; Hyperplasia - pathology; Image Processing, Computer-Assisted - methods; Immunohistochemistry; malignant breast lesions; Middle Aged; p53; Polycomb Repressive Complex 2 - metabolism; Precancerous Conditions - metabolism; Precancerous Conditions - pathology; quantitative image analysis; Retrospective Studies; Tumor Suppressor Protein p53 - metabolism
• ISSN: 8755-1039; 1097-0339
• DOI: 10.1002/dc.23217

Background Cytological differential diagnosis of atypical hyperplasia and well differentiated breast carcinoma may be challenging, because sometimes there is an overlap between the cytomorphological features of these lesions. The aim of the study was to investigate COX‐2, EZH‐2, p53 expression in carcinomas and the gray zone of breast cytology categories of atypical hyperplastic lesions with regard to biological behavior of the tumor. Methods FNA speciments from 100 patients with breast hyperplastic lesions and cancer were investigated by immunocytochemistry and a quantitative analysis for COX‐2, p53, and EZH‐2. Results Extent of staining for COX‐2 correlated with percentage of positive for EZH‐2 (P