Enhancement of mitochondrial function correlates with the extension of lifespan by caloric restriction and caloric restriction mimetics in yeast

• Published in: Biochemical and biophysical research communications 2013-11, Vol.441 (1), p.236-242
• Main Authors: Choi, Kyung-Mi, Lee, Hye-Lan, Kwon, Young-Yon, Kang, Mi-Sun, Lee, Sung-Keun, Lee, Cheol-Koo
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - metabolism; ATP; Caloric Restriction; Caloric restriction mimetics; Energy Metabolism - drug effects; Membrane Potential, Mitochondrial - drug effects; Mitochondria - drug effects; Mitochondria - metabolism; Mitochondrial membrane potential; Rapamycin; Reactive oxygen species; Reactive Oxygen Species - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Sirolimus - pharmacology; Time Factors
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2013.10.049

•Caloric restriction (CR) and rapamycin extended the chronological lifespan (CLS) of yeast.•Caffeine, curcumin, dapsone, metformin, resveratrol, and spermidine did not extend the CLS.•Rapamycin prevented total ROS, and increased MMP and ATP at the exponential phase.•CR reduced the mito-superoxide, and enhanced MMP and ATP at the stationary phase.•Rapamycin and CR worked via mitochondrial functions, but at different timeframes. Caloric restriction mimetics (CRMs) have been developed to mimic the effects of caloric restriction (CR). However, research reports for the effects of CRMs are often times inconsistent across different research groups. Therefore, in this study, we compared seven identified CRMs which extend the lifespans of various organisms including caffeine, curcumin, dapsone, metformin, rapamycin, resveratrol, and spermidine to CR for mitochondrial function in a single model, Saccharomyces cerevisiae. In this organism, rapamycin extended chronological lifespan (CLS), but other CRMs failed to extend CLS. Rapamycin enhanced mitochondrial function like CR did, but other CRMs did not. Both CR and rapamycin worked on mitochondrial function, but they worked at different windows of time during the chronological aging process.