Identification of mitogen-activated protein kinase kinase gene family and MKK–MAPK interaction network in maize

•9 Putative maize MKK genes have been identified.•4 New ZmMKK–ZmMPK interactions were identified using yeast two-hybrid.•Overexpression of ZmMKK4 enhanced oxidative stress tolerance in transgenic Arabidopsis. Plant mitogen-activated protein kinases (MAPK) are involved in important processes, includi...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2013-11, Vol.441 (4), p.964-969
Main Authors: Kong, Xiangpei, Pan, Jiaowen, Zhang, Dan, Jiang, Shanshan, Cai, Guohua, Wang, Li, Li, Dequan
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis
Arabidopsis thaliana
Brachypodium distachyon
Conserved Sequence
Expression analysis
Genes, Plant
Maize
MAP Kinase Signaling System
Mitogen-activated protein kinase
Mitogen-Activated Protein Kinase Kinases - classification
Mitogen-Activated Protein Kinase Kinases - genetics
Mitogen-Activated Protein Kinases - genetics
Mitogen-Activated Protein Kinases - metabolism
MKK
Molecular Sequence Data
Oryza sativa
Phylogenetic analysis
Phylogeny
Populus trichocarpa
Protein Interaction Maps
Two-Hybrid System Techniques
Zea mays
Zea mays - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•9 Putative maize MKK genes have been identified.•4 New ZmMKK–ZmMPK interactions were identified using yeast two-hybrid.•Overexpression of ZmMKK4 enhanced oxidative stress tolerance in transgenic Arabidopsis. Plant mitogen-activated protein kinases (MAPK) are involved in important processes, including stress signaling and development. MAPK kinases (MAPKK, MKK) have been investigated in several plant species including Arabidopsis thaliana, Oryza sativa, Populus trichocarpa, and Brachypodium distachyon. In the present study, nine putative maize MKK genes have been identified. Analysis of the conserved protein motifs, exon–intron junctions and intron phase has revealed high levels of conservation within the phylogenetic groups. Next, we defined four new ZmMKK–ZmMPK interactions using yeast two-hybrid. Finally, we examined the biological functions of the ZmMKK4 gene. Overexpression of ZmMKK4 in Arabidopsis conferred tolerance to oxidative stress by increased germination rate and early seedling growth compared with WT plants. Taken together, we provide a comprehensive bioinformatics analysis of the MKK gene family in maize genome and our data provide an important foundation for further functional study of MAPK and MKK families in maize.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2013.11.008