In vitro propagation of Ethiopian mustard (Brassica carinata A. BRAUN)

Brassica carinata (A. Braun) is an amphi-diploid species that originated from interspecific hybridization between Brassica nigra and Brassica oleracea in the highlands of Ethiopia. The crop has many desirable agronomic traits but with oil quality constraints like high erucic acid and glucosinolate c...

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Bibliographic Details
Published in:African journal of biotechnology 2014-11, Vol.13 (48), p.4438-4448
Main Authors: Getachew, Tafere Abrha, Firew, Mekbib, Belayneh, Admassu
Format: Article
Language:English
Subjects:
Brassica carinata
Brassica nigra
Brassica oleracea
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Summary:Brassica carinata (A. Braun) is an amphi-diploid species that originated from interspecific hybridization between Brassica nigra and Brassica oleracea in the highlands of Ethiopia. The crop has many desirable agronomic traits but with oil quality constraints like high erucic acid and glucosinolate contents. In this study, two genotypes and two types of explants were tested for callus induction, shoot and root regeneration in Murashige and Skoog (MS) medium under different concentrations of naphthalene acetic acid (NAA), 2, 4-dichlorophenoxyl acetic acid (2, 4-D), and 6-benzyl amino purine (BAP). The in vitro regeneration protocol developed can be used further undertaking of other tissue culture and genetic engineering work on B. carinata.
ISSN:1684-5315
1684-5315
DOI:10.5897/AJB2014.13819