Effect of Cryopreservation on the Sperm DNA Fragmentation Dynamics of the Bottlenose Dolphin (Tursiops truncatus)

Sperm DNA fragmentation is one of the major causes of infertility; the sperm chromatin dispersion test (SCDt) evaluates this parameter and offers the advantage of species‐specific validated protocol and ease of use under field conditions. The main purpose of this study was to evaluate sperm DNA frag...

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Bibliographic Details
Published in:Reproduction in domestic animals 2015-04, Vol.50 (2), p.227-235
Main Authors: Sánchez‐Calabuig, MJ, López‐Fernández, C, Johnston, SD, Blyde, D, Cooper, J, Harrison, K, Fuente, J, Gosálvez, J
Format: Article
Language:English
Subjects:
Animal reproduction
Animals
Aquatic mammals
Buffers
Cetacea
Chromatin
Cryopreservation
Cryopreservation - veterinary
Deoxyribonucleic acid
DNA
DNA Fragmentation
Dolphins
Dolphins & porpoises
Dolphins - physiology
Fragmentation
Fructose
humans
Incubation
Infertility
longevity
Male
Marine mammals
sampling
Semen
Semen Preservation - methods
Semen Preservation - veterinary
Sperm
Spermatozoa
Spermatozoa - physiology
Thawing
Tursiops truncatus
vegetables
Yolk
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Summary:Sperm DNA fragmentation is one of the major causes of infertility; the sperm chromatin dispersion test (SCDt) evaluates this parameter and offers the advantage of species‐specific validated protocol and ease of use under field conditions. The main purpose of this study was to evaluate sperm DNA fragmentation dynamics in both fresh and post‐thaw bottlenose dolphin sperm using the SCDt following different cryopreservation protocols to gain new information about the post‐thaw differential sperm DNA longevity in this species. Fresh and cryopreserved semen samples from five bottlenose dolphins were examined for sperm DNA fragmentation dynamics using the SCDt (Halomax®). Sperm DNA fragmentation was assessed immediately at collection and following cryopreservation (T0) and then after 0.5, 1, 4, 8, 24, 48 and 72 h incubation at 37°C. Serially collected ejaculates from four dolphins were frozen using different cryopreservation protocols in a TES‐TRIS‐fructose buffer (TTF), an egg‐yolk‐free vegetable lipid LP1 buffer (LP1) and human sperm preservation medium (HSPM). Fresh ejaculated spermatozoa initially showed low levels of DNA fragmentation for up to 48 h. Lower Sperm DNA fragmentation (SDF) was found in the second fresh ejaculate compared to the first when more than one sample was collected on the same day (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12474