Detecting genomic damages in the frog Dendropsophus minutus: preserved versus perturbed areas

The aim of the study was to use the comet assay (single-cell gel electrophoresis) and micronucleus test to assess the extent of genomic damage in the whole blood of Dendropsophus minutus from agroecosystems with great use of agrochemicals and to compare the results to those obtained from animals liv...

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Bibliographic Details
Published in:Environmental science and pollution research international 2015-03, Vol.22 (5), p.3947-3954
Main Authors: Gonçalves, M. W, Vieira, T. B, Maciel, N. M, Carvalho, W. F, Lima, L. S. F, Gambale, P. G, da Cruz, A. D, Nomura, F, Bastos, R. P, Silva, D. M
Format: Article
Language:English
Subjects:
Agricultural ecosystems
Agricultural pollution
Agriculture
Agrochemicals
agroecosystems
Analysis
Animals
Anura
Anura - genetics
Aquatic Pollution
Atmospheric Protection/Air Quality Control/Air Pollution
Bioassays
Biomonitoring
blood cells
Brazil
Comet Assay
Deoxyribonucleic acid
DNA
DNA Damage
Earth and Environmental Science
Ecosystems
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Environmental Monitoring - methods
Environmental Pollution - adverse effects
Environmental science
Frogs
gel electrophoresis
Genomics
Indicator organisms
Laboratories
Micronucleus Tests
Olea
Research Article
Studies
tadpoles
Toads
Waste Water Technology
Water Management
Water Pollution Control
Xenobiotics - adverse effects
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Summary:The aim of the study was to use the comet assay (single-cell gel electrophoresis) and micronucleus test to assess the extent of genomic damage in the whole blood of Dendropsophus minutus from agroecosystems with great use of agrochemicals and to compare the results to those obtained from animals living in unpolluted areas. Our results indicated that specimens of D. minutus collected in perturbed areas exhibited higher amounts of DNA damage in blood cells in comparison to animals from areas free of agricultural activities. The average and standard deviation of all comet assay parameters (tail length, percentage of DNA in the tail, and olive tail moment) and micronuclei frequency were significantly higher in specimens collected in perturbed areas than in the animals from preserved areas. Our study showed that animals from perturbed areas, such as agroecosystems, tend to have higher amounts of DNA damage than animals from reference areas. Moreover, we can conclude that D. minutus tadpoles could be included as a model organism in biomonitoring studies.
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-014-3682-1