Determination of dimetridazole residues in bovine tissue by liquid chromatography

An analytical procedure for the detection and identification of dimetridazole (DMZ) residues in muscle tissue is described. The tissue was homogenised with dichloromethane and the extract reduced to a small volume by rotary evaporation. The sample was further cleaned-up by solid phase extraction on...

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Bibliographic Details
Published in:Analytica chimica acta 1993-04, Vol.275 (1), p.317-321
Main Authors: Ramos, M., Aranda, A., Reuvers, Th, Jiménez, R.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Bovine tissue
Dimetridazole
General pharmacology
Liquid chromatography
Medical sciences
Muscle tissue
Pharmacology. Drug treatments
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Summary:An analytical procedure for the detection and identification of dimetridazole (DMZ) residues in muscle tissue is described. The tissue was homogenised with dichloromethane and the extract reduced to a small volume by rotary evaporation. The sample was further cleaned-up by solid phase extraction on silica cartridge and DMZ was eluted with ammonium acetate buffer (pH 4.3). The eluate was extracted with ethyl acetate and the organic phase evaporated in the presence of the mobile phase. DMZ was quantified and confirmed by reversed-phase liquid chromatography with UV-visible diode array spectrophotometric detection at 320 nm. The average recovery from spiked muscle was 84% in the concentration range 10–50 μg kg −1. The confirmation limit was calculated to be 5 μg kg −1.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(93)80308-8