Use of anion exchange perfusion chromatography for protease recovery at process scale

A protease from Aspergillus niger LCF 8 was purified in anion exchange mode using BioCAD Workstations and the process scaled up. The use of POROS 50 HQ media brings the benefits of Perfusion chromatography i.e. high resolution and high dynamic capacity with very short cycle times. Results obtained a...

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Bibliographic Details
Published in:Biotechnology techniques 1994-12, Vol.8 (12), p.895-900
Main Authors: BARTHOMEUF, C, POURRAT, H, BERTHON, J.-Y, AGIUS, D
Format: Article
Language:English
Subjects:
Aspergillus niger
Biological and medical sciences
Biotechnology
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Improved methods for extraction and purification of enzymes
Methods. Procedures. Technologies
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Summary:A protease from Aspergillus niger LCF 8 was purified in anion exchange mode using BioCAD Workstations and the process scaled up. The use of POROS 50 HQ media brings the benefits of Perfusion chromatography i.e. high resolution and high dynamic capacity with very short cycle times. Results obtained at pilot scale enable to purify at process scale 151g of protease/m super(3) crude broth in 1h. The small column size needed (2.25 I media/m super(3) crude broth) and the high processing speed of POROS* 50 HQ compared with conventional media lead to greatly improved cost effectiveness and higher product recovery.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF02447735