Nylon-linamarase electrode for rapid determination of linamarin

An enzyme electrode was constructed using cassava leaf linamarase covalently linked via polyethyleneimine to Hybond-N nylon. The nylon-enzyme electrode response was Nerstian for linamarin range of 0.1 to 20 mM. A steady state reading could be obtained within 4 to 6 mins. The nylon-enzyme discs could...

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Bibliographic Details
Published in:Biotechnology techniques 1993-10, Vol.7 (10), p.761-764
Main Author: Yeoh, H.H
Format: Article
Language:English
Subjects:
Biological and medical sciences
biosensors
Biotechnology
detection
electrodes
enzymes
Fundamental and applied biological sciences. Psychology
immobilization
Immobilization of enzymes and other molecules
Immobilization techniques
linamarin
Manihot
Manihot esculenta
Methods. Procedures. Technologies
nylon
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Summary:An enzyme electrode was constructed using cassava leaf linamarase covalently linked via polyethyleneimine to Hybond-N nylon. The nylon-enzyme electrode response was Nerstian for linamarin range of 0.1 to 20 mM. A steady state reading could be obtained within 4 to 6 mins. The nylon-enzyme discs could be reused. Compared to the previously reported enzyme electrode prepared by entrappment of linamarase in ENT-4000 prepolymer resins, the nylon-enzyme electrode gave faster response and could save analysis time by 60%.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00152627