Rapid screening method for ivermectin residue detection in cattle muscle and liver by liquid chromatography with UV detection

A rapid screening method for the determination of residue of ivermectin in edible bovine tissue and liver is described. The analyte is extracted from the tissue with a methanol-water mixture. The clean-up procedure is based on solid phase extraction on a Sep-Pak C 18 cartridge. Analysis is performed...

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Bibliographic Details
Published in:Analytica chimica acta 1993-04, Vol.275 (1), p.353-358
Main Authors: Reuvers, Th, Diaz, R., Martin de Pozuelo, M., Ramos, M.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Bovine tissue
Cattle
General pharmacology
Ivermectin
Liquid chromatography
Liver tissue
Medical sciences
Muscle tissue
Pharmacology. Drug treatments
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Summary:A rapid screening method for the determination of residue of ivermectin in edible bovine tissue and liver is described. The analyte is extracted from the tissue with a methanol-water mixture. The clean-up procedure is based on solid phase extraction on a Sep-Pak C 18 cartridge. Analysis is performed by isocratic elution with methanolacetonitrile-water (9:9:2, v/v/v) on a Novapak C 18 5-μm column and UV-diode array spectrophotometric detection at 254 nm. The average recovery from spiked bovine muscle was 78.6% (S.D. = 15.0%) and from spiked liver sample 78.5% (S.D. = 10.9%) in the concentration range 14–275 μg kg −1. The method has a detection limit of 5–10 μg kg −1.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(93)80314-B