Measurement of antiphospholipid antibody by ELISA using purified beta sub(2)-glycoprotein I in preeclampsia

• Published in: Clinical and experimental immunology 1993-01, Vol.94 (1), p.196-200
• Main Authors: Yamamoto, T, Yoshimura, S, Geshi, Y, Sasamori, Y, Okinaga, S, Kobayashi, T, Mori, H
• Format: Article
• Language: English
• ISSN: 0009-9104

It has been reported that the binding of some antiphospholipid antibodies (APA) to phospholipids requires the presence of beta sub(2)-glycoprotein I ( beta sub(2)-GPI). Using a new ELISA, in which well coated phospholipids were treated with a constant amount of purified beta sub(2)-GPI, we tried to detect the presence of APA which binds to phospholipid/ beta sub(2)-GPI complex or to phospholipids such as cardiolipin (CA) and phosphatidylserine (PS) in preeclampsia, and to check for clinical abnormalities in antibody-positive cases. Serum samples were taken from 43 cases of preeclampsia, including 26 cases of the severe type, and 47 normal pregnant women. Positive rates of anticardiolipin antibody (ACA) by ELISA using CA/ beta sub(2)-GPI complex in mild, severe and total preeclampsia were 20.0%, 17.4% and 18.4% respectively. No antibody-positive cases were found in normal pregnancies. The frequency of intrauterine growth retardation (IUGR) in the ACA-positive subjects was higher than that of ACA negatives. We suggest that the ACA and APSA which bind to phospholipid/ beta sub(2)-GPI complex are detectable in preeclampsia, and that these antiphospholipid antibodies are related to fetal growth.