RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise

Highlights • EDNAP organized a collaborative exercise on RNA/DNA co-analysis of skin samples. • 8 skin and 3 HKG markers were tested for specificity, sensitivity and applicability. • LCE1C, LOR and B2M were detected in most contact traces, detection of the other markers was inconsistent. • LCE1C and...

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Published in:Forensic science international : genetics 2015-05, Vol.16, p.139-147
Main Authors: Haas, C, Hanson, E, Banemann, R, Bento, A.M, Berti, A, Carracedo, Á, Courts, C, Cock, G. De, Drobnic, K, Fleming, R, Franchi, C, Gomes, I, Hadzic, G, Harbison, S.A, Hjort, B, Hollard, C, Hoff-Olsen, P, Keyser, C, Kondili, A, Maroñas, O, McCallum, N, Miniati, P, Morling, N, Niederstätter, H, Noël, F, Parson, W, Porto, M.J, Roeder, A.D, Sauer, E, Schneider, P.M, Shanthan, G, Sijen, T, Syndercombe Court, D, Turanská, M, van den Berge, M, Vennemann, M, Vidaki, A, Zatkalíková, L, Ballantyne, J
Format: Article
Language:English
Subjects:
Contact traces
DNA - analysis
EDNAP exercise
Forensic Genetics
Forensic science
Humans
mRNA profiling
Pathology
RNA - analysis
RNA/DNA co-extraction
Skin
Skin - chemistry
Tissue identification
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Summary:Highlights • EDNAP organized a collaborative exercise on RNA/DNA co-analysis of skin samples. • 8 skin and 3 HKG markers were tested for specificity, sensitivity and applicability. • LCE1C, LOR and B2M were detected in most contact traces, detection of the other markers was inconsistent. • LCE1C and LOR markers showed some cross-reactivity with non-skin sample types. • With contact traces the method reaches the detection limit.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2015.01.002