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RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise
Highlights • EDNAP organized a collaborative exercise on RNA/DNA co-analysis of skin samples. • 8 skin and 3 HKG markers were tested for specificity, sensitivity and applicability. • LCE1C, LOR and B2M were detected in most contact traces, detection of the other markers was inconsistent. • LCE1C and...
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| Published in: | Forensic science international : genetics 2015-05, Vol.16, p.139-147 |
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| creator | Haas, C Hanson, E Banemann, R Bento, A.M Berti, A Carracedo, Á Courts, C Cock, G. De Drobnic, K Fleming, R Franchi, C Gomes, I Hadzic, G Harbison, S.A Hjort, B Hollard, C Hoff-Olsen, P Keyser, C Kondili, A Maroñas, O McCallum, N Miniati, P Morling, N Niederstätter, H Noël, F Parson, W Porto, M.J Roeder, A.D Sauer, E Schneider, P.M Shanthan, G Sijen, T Syndercombe Court, D Turanská, M van den Berge, M Vennemann, M Vidaki, A Zatkalíková, L Ballantyne, J |
| description | Highlights • EDNAP organized a collaborative exercise on RNA/DNA co-analysis of skin samples. • 8 skin and 3 HKG markers were tested for specificity, sensitivity and applicability. • LCE1C, LOR and B2M were detected in most contact traces, detection of the other markers was inconsistent. • LCE1C and LOR markers showed some cross-reactivity with non-skin sample types. • With contact traces the method reaches the detection limit. |
| doi_str_mv | 10.1016/j.fsigen.2015.01.002 |
| format | article |
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De ; Drobnic, K ; Fleming, R ; Franchi, C ; Gomes, I ; Hadzic, G ; Harbison, S.A ; Hjort, B ; Hollard, C ; Hoff-Olsen, P ; Keyser, C ; Kondili, A ; Maroñas, O ; McCallum, N ; Miniati, P ; Morling, N ; Niederstätter, H ; Noël, F ; Parson, W ; Porto, M.J ; Roeder, A.D ; Sauer, E ; Schneider, P.M ; Shanthan, G ; Sijen, T ; Syndercombe Court, D ; Turanská, M ; van den Berge, M ; Vennemann, M ; Vidaki, A ; Zatkalíková, L ; Ballantyne, J</creator><creatorcontrib>Haas, C ; Hanson, E ; Banemann, R ; Bento, A.M ; Berti, A ; Carracedo, Á ; Courts, C ; Cock, G. De ; Drobnic, K ; Fleming, R ; Franchi, C ; Gomes, I ; Hadzic, G ; Harbison, S.A ; Hjort, B ; Hollard, C ; Hoff-Olsen, P ; Keyser, C ; Kondili, A ; Maroñas, O ; McCallum, N ; Miniati, P ; Morling, N ; Niederstätter, H ; Noël, F ; Parson, W ; Porto, M.J ; Roeder, A.D ; Sauer, E ; Schneider, P.M ; Shanthan, G ; Sijen, T ; Syndercombe Court, D ; Turanská, M ; van den Berge, M ; Vennemann, M ; Vidaki, A ; Zatkalíková, L ; Ballantyne, J</creatorcontrib><description>Highlights • EDNAP organized a collaborative exercise on RNA/DNA co-analysis of skin samples. • 8 skin and 3 HKG markers were tested for specificity, sensitivity and applicability. • LCE1C, LOR and B2M were detected in most contact traces, detection of the other markers was inconsistent. • LCE1C and LOR markers showed some cross-reactivity with non-skin sample types. • With contact traces the method reaches the detection limit.</description><identifier>ISSN: 1872-4973</identifier><identifier>EISSN: 1878-0326</identifier><identifier>DOI: 10.1016/j.fsigen.2015.01.002</identifier><identifier>PMID: 25600397</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Contact traces ; DNA - analysis ; EDNAP exercise ; Forensic Genetics ; Forensic science ; Humans ; mRNA profiling ; Pathology ; RNA - analysis ; RNA/DNA co-extraction ; Skin ; Skin - chemistry ; Tissue identification</subject><ispartof>Forensic science international : genetics, 2015-05, Vol.16, p.139-147</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2015 Elsevier Ireland Ltd</rights><rights>Copyright © 2015 Elsevier Ireland Ltd. 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| ispartof | Forensic science international : genetics, 2015-05, Vol.16, p.139-147 |
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| source | ScienceDirect Journals |
| subjects | Contact traces DNA - analysis EDNAP exercise Forensic Genetics Forensic science Humans mRNA profiling Pathology RNA - analysis RNA/DNA co-extraction Skin Skin - chemistry Tissue identification |
| title | RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise |
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