Green tea changes serum and liver metabolomic profiles in mice with high‐fat diet‐induced obesity

• Published in: Molecular nutrition & food research 2015-04, Vol.59 (4), p.784-794
• Main Authors: Lee, Lan‐Sook, Choi, Ji Hea, Sung, Mi Jeong, Hur, Jin‐Young, Hur, Haeng Jeon, Park, Jong‐Dae, Kim, Young‐Chan, Gu, Eun‐Ji, Min, Byungjin, Kim, Hyun‐Jin
• Format: Article
• Language: English
• Subjects: Acetyl-CoA acyltransferase 2; Acetyl-CoA C-Acyltransferase - metabolism; Acyl-CoA Dehydrogenase - metabolism; adipose tissue; Adipose Tissue - metabolism; animal disease models; Animals; beta oxidation; Blood Glucose - metabolism; blood serum; carnitine; Carnitine O-Palmitoyltransferase - metabolism; Cholesterol - blood; Diet, High-Fat - adverse effects; Dietary Fats - administration & dosage; Energy Metabolism; enzymes; Fatty acid β-oxidation; flavanols; glucose; Green tea; high fat diet; insulin; Insulin - blood; leptin; Leptin - blood; Lipid Metabolism; liver; Liver - metabolism; Male; metabolites; Metabolome; Metabolomics; Mice; Mice, Inbred C57BL; Mice, Obese; Mitochondria - drug effects; Mitochondria - metabolism; Multivariate Analysis; Obesity; Obesity - diet therapy; Obesity - etiology; Tea - chemistry; triacylglycerols; Triglycerides - blood
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.201400470

SCOPE: Green tea (GT) consumption helps to prevent and control obesity by stimulating hepatic lipid metabolism. However, GT‐induced changes in serum and liver metabolomes associated with the anti‐obesity effects are not clearly understood. The aim of this study was to identify and validate metabolomic profiles in the livers and sera of GT‐fed obese mice to elucidate the relationship between GT consumption and obesity prevention. METHODS AND RESULTS: Serum and liver metabolites were analyzed in mice fed normal diet, high‐fat diet (HFD), HFD with GT, and HFD with crude catechins, using LC‐quadrupole TOF MS. The addition of 1% GT to HFD reduced adipose tissue and the levels of blood triglycerides, glucose, insulin, and leptin elevated in HFD‐fed mice. We proposed an HFD‐induced obesity pathway and validated it by investigating the key regulatory enzymes of mitochondrial β‐oxidation: carnitine palmitoyltransferase‐1 and ‐2, acyl‐coenzyme A dehydrogenase, and acetyl‐coenzyme A acyltransferase. The results showed that HFD‐induced abnormal mitochondrial β‐oxidation was moderated by the consumption of caffeine‐ and theanine‐enriched GT. CONCLUSION: Results of LC/MS‐based metabolomic analysis of obese mice showed changes associated with abnormal lipid and energy metabolism, which were alleviated by GT intake, indicating the mechanism underlying the anti‐obesity effects of GT.