Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO(2-x) NPs

Cerium Oxide nanoparticles (CeO(2-x) NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO(2-x) NPs are fluore...

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Bibliographic Details
Published in:Nanoscale 2015-04, Vol.7 (15), p.6588-6598
Main Authors: Qiu, Yuan, Rojas, Elena, Murray, Richard A, Irigoyen, Joseba, Gregurec, Danijela, Castro-Hartmann, Pablo, Fledderman, Jana, Estrela-Lopis, Irina, Donath, Edwin, Moya, Sergio E
Format: Article
Language:English
Subjects:
Apoptosis
Cell Lineage
Cell Separation
Cerium - chemistry
Colloids - chemistry
Flow Cytometry
Fluorescein-5-isothiocyanate - chemistry
HEK293 Cells
Humans
Mass Spectrometry
Metal Nanoparticles - chemistry
Methacrylates - chemistry
Microscopy, Confocal
Microscopy, Electron, Transmission
Polymers - chemistry
Reactive Oxygen Species - chemistry
Spectrum Analysis, Raman
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Summary:Cerium Oxide nanoparticles (CeO(2-x) NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO(2-x) NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO(2-x) NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO(2-x) NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO(2-x) NPs. The brush coating does not prevent CeO(2-x) NPs from displaying antioxidant properties.
ISSN:2040-3372
DOI:10.1039/c5nr00884k