Development of a paper-type tyrosinase biosensor for detection of phenolic compounds

A low‐cost, portable, and disposable paper‐type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper‐strip absorption method. Tyrosinase and a chromophore (3‐methyl‐2‐benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, whic...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2015-01, Vol.62 (1), p.132-136
Main Authors: Senyurt, Özge, Eyidoan, Fusun, Yilmaz, Remziye, Oz, M Tufan, Ozalp, V Cengiz, Arica, Yakup, Oktem, Huseyin A
Format: Article
Language:English
Subjects:
4-chlorophenol
Agaricales - enzymology
Benzothiazoles - chemistry
Biochemistry
Biosensing Techniques - methods
Biosensors
Biotechnology
catechol
Chlorophenol
Chromophores
cresol
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
Hydrazones - chemistry
Kinetics
l-dopamine
Limit of Detection
Monitoring
Monophenol Monooxygenase - chemistry
Monophenol Monooxygenase - metabolism
paper strip
phenol
Phenols
Phenols - analysis
Phenols - chemistry
Portability
Stability
Tyrosinase
Waste water
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Summary:A low‐cost, portable, and disposable paper‐type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper‐strip absorption method. Tyrosinase and a chromophore (3‐methyl‐2‐benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l‐dopamine). The biosensor was responsive to phenolic compounds such as 4‐chlorophenol, catechol, m‐cresol, and p‐cresol. The sensor showed stability for 70 days. The developed biosensor can be used for remote on‐site qualitative monitoring of phenolic compounds in wastewater samples.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.1246