Low-temperature plasma-induced antiproliferative effects on multi-cellular tumor spheroids

Biomedical applications of low-temperature plasmas are of growing interest, especially in the field of plasma-induced anti-tumor effects. The present work is aimed at investigating the regionalized antiproliferative effects of low-temperature plasmas on a multicellular tumor spheroid (MCTS), a model...

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Bibliographic Details
Published in:New journal of physics 2014-04, Vol.16 (4), p.43027-20
Main Authors: Plewa, Joseph-Marie, Yousfi, Mohammed, Frongia, Céline, Eichwald, Olivier, Ducommun, Bernard, Merbahi, Nofel, Lobjois, Valérie
Format: Article
Language:English
Subjects:
Air conditioners
Anticancer properties
antiproliferative effect
Antiproliferatives
Biomedical materials
Cancer
Cell death
Colon
Damage accumulation
Damage detection
Engineering Sciences
genotoxic and cytotoxic effects
Genotoxicity
Histones
Low temperature
low temperature plasma
Media
multi-cellular tumor spheroid
Phosphorylation
Physics
Plasma jets
Plasmas
Plasmas (physics)
reactive oxygen species
Spheroids
Three dimensional
Three dimensional models
Tumors
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Summary:Biomedical applications of low-temperature plasmas are of growing interest, especially in the field of plasma-induced anti-tumor effects. The present work is aimed at investigating the regionalized antiproliferative effects of low-temperature plasmas on a multicellular tumor spheroid (MCTS), a model that mimics the 3D organization and regionalization of a microtumor region. We report that a low-temperature plasma jet, using helium flow in open air, inhibits HCT116 colon carcinoma MCTS growth in a dose-dependent manner. This growth inhibition is associated with the loss of Ki67, and the regionalized accumulation of DNA damage detected by histone H2AX phosphorylation. This regionalized genotoxic effect leads to massive cell death and loss of the MCTS proliferative region. The use of reactive oxygen species (ROS), scavenger N-acetyl cysteine (NAC) and plasma-conditioned media demonstrate that the ROS generated in the media after exposure to low-temperature plasma play a major role in these observed effects. These findings strengthen the interest in the use of MCTS for the evaluation of antiproliferative strategies, and open new perspectives for studies dedicated to demonstrate the potential of low-temperature plasma in cancer therapy.
ISSN:1367-2630
1367-2630
DOI:10.1088/1367-2630/16/4/043027