Simultaneous pretreatment and saccharification: green technology for enhanced sugar yields from biomass using a fungal consortium

Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow,...

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Bibliographic Details
Published in:Bioresource technology 2015-03, Vol.179, p.50-57
Main Authors: Dhiman, Saurabh Sudha, Haw, Jung-Rim, Kalyani, Dayanand, Kalia, Vipin C, Kang, Yun Chan, Lee, Jung-Kul
Format: Article
Language:English
Subjects:
Biofuels
Biomass
Biotechnology - methods
Carbohydrate Metabolism
Carbohydrates - biosynthesis
Consortia
Ethanol - metabolism
Fermentation
Fungi
Fungi - metabolism
Green Chemistry Technology - methods
Hydrolysis
Microbial Consortia
Microscopy, Atomic Force
Oryza - chemistry
Oryza sativa
Phenols - analysis
Pretreatment
Saccharification
Saccharomyces cerevisiae - isolation & purification
Saccharomyces cerevisiae - metabolism
Solubility
Spark plasma sintering
Straw
Sugars
Surface-Active Agents - pharmacology
Waste Products - analysis
Willow
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Summary:Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow, respectively. Hydrolysis of soaked substrates using a newly developed fungal consortium resulted in saccharification yield of up to 74.2% and 63.6% for rice straw and willow, respectively. A high saccharification yield was obtained with soaked rice straw and willow without using any hazardous chemicals. The efficiency of each step related to SPS was confirmed by atomic force microscopy. The suitability of the developed SPS process was further confirmed by converting the hydrolysate from the process into bioethanol with 72.4% sugar conversion efficiency. To the best of our knowledge, this is the first report on the development of a less tedious, single-pot, and eco-friendly SPS methodology.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2014.11.059