Oleic and linoleic fatty acids downregulate Slc2a4/GLUT4 expression via NFKB and SREBP1 in skeletal muscle cells

• Published in: Molecular and cellular endocrinology 2015-02, Vol.401, p.65-72
• Main Authors: Poletto, Ana Cláudia, Furuya, Daniela Tomie, David-Silva, Aline, Ebersbach-Silva, Patrícia, Lellis Santos, Camilo, Corrêa-Giannella, Maria Lúcia, Passarelli, Marisa, Machado, Ubiratan Fabres
• Format: Article
• Language: English
• Subjects: Animals; Binding; Cell Nucleus - metabolism; Cells, Cultured; Down-Regulation - drug effects; Electrophoretic mobility shift assay; Fatty acids; Genes; Glucose Transporter Type 4 - genetics; Glucose Transporter Type 4 - metabolism; Inhibitors; Insulin; Insulin resistance; Linoleic Acid - pharmacology; LXR-α; Muscles; Myoblasts, Skeletal - cytology; Myoblasts, Skeletal - metabolism; NF-kappa B - genetics; Oleic Acid - pharmacology; PPAR-γ; Promoter Regions, Genetic; Proteins; Rats; Regulations; Regulators; Sterol Regulatory Element Binding Protein 1 - genetics; Transcription factors; Unsaturated fatty acid
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/j.mce.2014.12.001

•Oleic and linoleic acids repress Slc2a4 gene expression in skeletal muscle cell.•Skeletal muscle cell GLUT4 content decreases by oleic and linoleic acids.•SREBP1 binding activity into Slc2a4 gene decreases by oleic and linoleic acids.•Oleic and linoleic acids increase proteins binding into Slc2a4-NFKB binding site.•Phosphorylation of IKKα/β increases by oleic and linoleic acids. Oleic (OA) and linoleic (LA) fatty acids may be important regulators of Slc2a4 gene (GLUT4 protein) in skeletal muscle, thus participating in insulin resistance. We investigated the effect of OA and LA on the Slc2a4/GLUT4 expression in L6 muscle cells; as well as potential transcriptional regulators. OA and LA (50–400 µM) decreased the Slc2a4/GLUT4 expression in a dose-dependent way (maximum of ~50%, P