Nucleic acid spot hybridization based species-specific detection of Sclerotium rolfsii associated with collar rot disease of Amorphophallus paeoniifolius

Collar rot is one of the most destructive and prevalent disease of Amorphophallus paeoniifolius, resulting in heavy yield losses. The causative organism, Sclerotium rolfsii is a soil-borne polyphagous fungus characterized by prolific growth and ability to produce persistent sclerotia. The pathogen p...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2015-02, Vol.31 (2), p.315-320
Main Authors: Pravi, V, Jeeva, M. L, Archana, P. V
Format: Article
Language:English
Subjects:
Acids
Agriculture
Amorphophallus - microbiology
Amorphophallus paeoniifolius
Analysis
Applied Microbiology
Ascomycota - classification
Ascomycota - genetics
Athelia rolfsii
Biochemistry
Biomedical and Life Sciences
Biotechnology
Collars
Crop diseases
Crops
Deoxyribonucleic acid
detection limit
Disease management
DNA
DNA Probes - analysis
Environmental Engineering/Biotechnology
Flowers & plants
fungi
Genetic testing
Hybridization
inoculum
Life Sciences
microbial detection
Microbiology
nucleic acid hybridization
Nucleic Acid Hybridization - methods
Nucleic acids
Original Paper
pathogen identification
Pathogens
Plant diseases
Plant Diseases - microbiology
Planting
Ribosomal DNA
sclerotia
Sclerotium rolfsii
Sensors
soil
Soil (material)
Soil contaminants
Soil Microbiology
Species Specificity
Spots
Studies
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Description
Summary:Collar rot is one of the most destructive and prevalent disease of Amorphophallus paeoniifolius, resulting in heavy yield losses. The causative organism, Sclerotium rolfsii is a soil-borne polyphagous fungus characterized by prolific growth and ability to produce persistent sclerotia. The pathogen propagules surviving in soil and planting material are the major sources of inoculum. This study presents the suitability of DNA hybridization technique for species specific detection of S. rolfsii in soil and planting material. The detection limit of the probe was 10–15 pg of pure pathogen DNA. The developed probe was found to be highly specific and could be used for accurate identification of pathogen up to the species level. The protocol was standardized for detection of the pathogen in naturally infected field samples.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-014-1783-0