A novel folic acid-conjugated TiO2–SiO2 photosensitizer for cancer targeting in photodynamic therapy

•A novel folic acid-conjugated TiO2–SiO2 nano-photosensitizer was synthesized for targeted photo-killing of cancer cells.•High photodynamic reactivity, improved cell internalization and cellular and blood compatibility were well demonstrated.•A strong photo-killing effect on the human nasopharyngeal...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2015-01, Vol.125, p.197-205
Main Authors: Feng, Xiaohui, Zhang, Shaokun, Wu, Hong, Lou, Xia
Format: Article
Language:English
Subjects:
Animals
Biological Transport
Cancer
Cell Line
Fibroblasts
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - radiation effects
Folic acid
Folic Acid - chemistry
Human
Humans
KB Cells
Mathematical analysis
Mice
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Photochemotherapy
Photodynamic therapy
Photosensitizer, Photo-killing
Photosensitizing Agents - chemical synthesis
Photosensitizing Agents - pharmacology
Silicon Dioxide - chemistry
Targeting
Titanium - chemistry
Titanium dioxide
Titanium dioxide nanoparticles
Ultraviolet Rays
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Summary:•A novel folic acid-conjugated TiO2–SiO2 nano-photosensitizer was synthesized for targeted photo-killing of cancer cells.•High photodynamic reactivity, improved cell internalization and cellular and blood compatibility were well demonstrated.•A strong photo-killing effect on the human nasopharyngeal epidermoid cancer (KB) cells was achieved. In this paper, a novel folic acid-conjugated silica-coated titanium dioxide (TiO2–SiO2) photosensitizer was synthesized and characterized using various analytical instruments. The photosensitizer was further assessed with regards to its photoreactivity, cellular and hemocompatibility, cell internalization, and phototoxicity. Conjugating folic acid with TiO2–SiO2 has shown a significantly improved compatibility of the nanoparticles with the mouse fibroblast cells (L929) at 24h. An improved compatibility with the human nasopharyngeal epidermoid cancer (KB) cells was also demonstrated, but to a slightly reduced degree. Enhanced cell internalization was well demonstrated in the TiO2–SiO2 folate nanoparticles. Upon exposure to UV light, TiO2–SiO2 folate nanoparticles maintained a high level photodynamic reactivity and yielded a 38–43% photo-killing of KB cells. The photo-killing effect increased with increasing dosage in the investigated concentration range of 50–100μgml−1.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2014.11.035