The identification of Bradyrhizobium japonicum strains isolated from Italian soils

• Published in: Microbiology (Society for General Microbiology) 1994-09, Vol.140 (9), p.2333-2339
• Main Authors: Kay, H. E, Coutinho, H. L. C, Fattori, M, Manfio, G. P, Goodacre, R, Nuti, M. P, Basaglia, M, Beringer, J. E
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Bradyrhizobium japonicum; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Microbiology; Pest resistance; Plant pathogens; Varietal selection. Specialized plant breeding, plant breeding aims
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/13500872-140-9-2333

1 School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK 2 ICI Seeds - SES NV/SA, Industriepark 15, B-3300 Tienen, Belgium 3 Department of Microbiology, University of Newcastle-upon-Tyne, The Medical School, Framlington Place, Newcastle-upon-Tyne NE2 4HH, UK 4 Department of Biological Sciences, University of Wales, Aberystwyth, Dyfed SY23 3DA, UK 5 Dipartimento di Biotecnologie Agrarie, Universita di Padova, Via Gradenigo, 6-35131 Padova, Italy 6 Heligenetics SpA, Via Provinciale 12, Gaiba, Rovigo, Italy ABSTRACT Pyrolysis mass spectrometry (PYMS) and PCR using arbitrary primers were used to characterize strains of Bradyrhizobium japonicum isolated from fields in northern Italy. The combination of techniques allowed us to identify bacteria derived from inoculants and to demonstrate that information about the nature of one inoculant was incorrect. PYMS also indicated that the derivatives from one inoculant formed two distinct populations: one like the parent strain and the other altered phenotypically. Author for correspondence: John E. Beringer. Tel: [phone]. Fax: [phone]. Keywords: Bradyrhizobium japonicum , pyrolysis mass spectrometry, polymerase chain reaction, strain identification, soil bacteria Present address: Fundação Tropical de Pesquisas e Technologia André Tosello, Rua Latino Coelho 1301, Campinas-SP 13087-010, Brazil.