C. elegans as a model to study PTEN’s regulation and function

PTEN (phosphatase and tensin homolog deleted on chromosome 10) has important roles in tumor suppression, metabolism, and development, yet its regulators, effectors, and functions are not fully understood. DAF-18 is the PTEN ortholog in Caenorhabditis elegans. DAF-18’s role is highly conserved to hum...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2015-05, Vol.77-78, p.180-190
Main Authors: Liu, Jun, Chin-Sang, Ian D.
Format: Article
Language:English
Subjects:
Animals
C. elegans
Caenorhabditis elegans - physiology
Caenorhabditis elegans Proteins - physiology
DAF-18
Genetics
Humans
Models, Animal
PTEN
PTEN Phosphohydrolase - physiology
Tumor suppressor
Tumor Suppressor Proteins - physiology
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Summary:PTEN (phosphatase and tensin homolog deleted on chromosome 10) has important roles in tumor suppression, metabolism, and development, yet its regulators, effectors, and functions are not fully understood. DAF-18 is the PTEN ortholog in Caenorhabditis elegans. DAF-18’s role is highly conserved to human PTEN, and can be functionally replaced by human PTEN. Thus C. elegans provides a valuable model to study PTEN. This review assesses current and emerging methods to study DAF-18’s regulators and functions in C. elegans. We propose genetic modify screens to identify genes that interact with daf-18/PTEN. These genes are potential targets for anticancer drug therapies. We also provide a review on the roles DAF-18/PTEN has during C. elegans development and how studying these physiological roles can provide mechanistic insight on DAF-18/PTEN function.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2014.12.009