Influence of maternal diet enrichment with conjugated linoleic acids on lipoxygenase metabolites of polyunsaturated fatty acids in serum of their offspring with 7,12-dimethylbenz[a]anthracene induced mammary tumors

• Published in: Prostaglandins & other lipid mediators 2015-01, Vol.116-117, p.10-18
• Main Authors: Białek, Agnieszka, Jelińska, Małgorzata, Tokarz, Andrzej
• Format: Article
• Language: English
• Subjects: 9,10-Dimethyl-1,2-benzanthracene - toxicity; Animals; Breast cancer; Carcinogens - toxicity; Conjugated linoleic acids; Female; HEPE; HETE; HODE; Linoleic Acids, Conjugated - pharmacokinetics; Linoleic Acids, Conjugated - pharmacology; Lipoxygenase; Lipoxygenase - metabolism; Mammary Neoplasms, Experimental - chemically induced; Mammary Neoplasms, Experimental - metabolism; Mammary Neoplasms, Experimental - pathology; Mammary Neoplasms, Experimental - prevention & control; Pregnancy; Rats; Rats, Sprague-Dawley
• ISSN: 1098-8823
• DOI: 10.1016/j.prostaglandins.2014.10.001

•Effect of CLA in diet of female rats on cancer risk in progeny rats was studied.•PUFA and their LOX metabolites in serum were analyzed.•CLA in diet of rats correspond with lower risk of mammary tumors in their offspring.•CLA compete with PUFA and influence serum concentration of their LOX metabolites.•Competition of CLA with PUFA can partly explain the anticancerogenic action of CLA. Conjugated linoleic acids (CLA), which are a group of naturally occurring in food isomers of linoleic acid, seem to be active in each step of cancer development. There are many possible mechanisms of this action, and interactions with polyunsaturated fatty acids (PUFA) in lipoxygenase (LOX) and cyclooxygenase (COX) pathways are among the most likely ones. The aim of this study was to assess the influence of diet supplementation with CLA of pregnant and breastfeeding Sprague-Dawley female rats on selected polyunsaturated fatty acids and their LOX metabolites concentrations in serum of the progeny with chemically induced mammary tumors. We confirmed that higher supply of CLA in the diet of female rats corresponded with the lower susceptibility to chemically induced mammary tumors in their female offspring. It also influenced the polyunsaturated n-3 and n-6 fatty acid concentrations in serum, as well as the concentrations of their LOX metabolites. The significant negative correlation between the concentrations of two CLA isomers in serum and linoleic acid (p=0.0144, p=0.0098), eicosapentaenoic acid (p=0.0158, p=0.0124), and 5-HEPE (p=0.0014, p=0.01690) and between cis-9, trans-11 CLA and 15-HEPE was detected, whereas arachidonic acid concentration positively correlated with CLA concentration in serum (p=0.0150, p=0.0231). Our results indicate that CLA can compete with PUFA and influence serum concentration of PUFA and their LOX metabolites, which could partly explain the anticancerogenic action of CLA.