Effect of phosphate limitation on synthesis of periplasmic cyclic beta-(1,2)-glucans

Rhizobium meliloti and Agrobacterium tumefaciens synthesize periplasmic cyclic beta(1,2)-glucans during adaptation to hypoosmotic environments. It also appears that these glucans provide important functions during the interactions of these bacteria with plant hosts. A large fraction of these glucans...

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Bibliographic Details
Published in:Applied and environmental microbiology 1995-03, Vol.61 (3), p.1045-1053
Main Authors: Breedveld, M.W. (Wageningen Agricultural University, Wageningen, The Netherlands), Benesi, A.J, Marco, M.L, Miller, K.J
Format: Article
Language:English
Subjects:
ACIDE SUCCINIQUE
ACIDO SUCCINICO
AGROBACTERIUM TUMEFACIENS
Bacteria
Bacteriology
Biochemistry
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
COMPOSE ORGANOPHOSPHORE
COMPUESTO ORGANICO DEL FOSFORO
DISPONIBILIDAD DE NUTRIENTES
DISPONIBILITE D'ELEMENT NUTRITIF
FOSFORO
Fundamental and applied biological sciences. Psychology
GLICEROL
GLUCANE
GLUCANO
GLYCEROL
Metabolism. Enzymes
METABOLISME DES GLUCIDES
METABOLISMO DE CARBOHIDRATOS
Microbiology
Mission oriented research
PHOSPHORE
Physiology and metabolism
POLISACARIDOS
POLYHOLOSIDE
REACCIONES QUIMICAS
REACTION CHIMIQUE
RHIZOBIUM MELILOTI
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Summary:Rhizobium meliloti and Agrobacterium tumefaciens synthesize periplasmic cyclic beta(1,2)-glucans during adaptation to hypoosmotic environments. It also appears that these glucans provide important functions during the interactions of these bacteria with plant hosts. A large fraction of these glucans may become modified with anionic substituents such as phosphoglycerol or succinic acid; however, the role(s) of these substituents is unknown. In this study, we show that growth of these bacteria in phosphate-limited media leads to a dramatic reduction in the levels of phosphoglycerol substituents present on the periplasmic cyclic beta(1,2)-glucans. Under these growth conditions, R. meliloti 1021 was found to synthesize anionic cyclic beta-(1,2)-glucans containing only succinic acid substituents. Similar results were obtained with R. meliloti 7154 (an exoH mutant which lacks the ability to succinylate its high-molecular-weight exopolysaccharide), revealing that succinylation of the cyclic beta-(1,2)-glucans is mediated by an enzyme system distinct from that involved in the succinylation of exopolysaccharide. In contrast, when A. tumefaciens C58 was grown in a phosphate-limited medium, it was found to synthesize only neutral cyclic beta-(1,2)-glucans
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.61.3.1045-1053.1995