Daily administration times of Canhuang tablet based on a pharmacodynamic/pharmacokinetic model in jaundiced rats

OBJECTIVE： To study the daily administration times of Canhuang tablet （CHT） for treating jaundice in rats based on a pharmacodynamic/pharma- cokinetic model. METHODS： Rats were modeled by 4% 1-Naphthylisothiocyanate （75 mg/kg, p.o.）. After 48 h, CHT was given （p.o.） at 0.75 g/kg once a day, 0.375 g/...

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Bibliographic Details
Published in:Journal of traditional Chinese medicine 2015-02, Vol.35 (1), p.84-89
Main Authors: Jin, Han, Baode, Shen, He, Xu, Ling, Dai, Hailong, Yuan
Format: Article
Language:English
Subjects:
Animals
Bilirubin - metabolism
Disease Models, Animal
Drugs, Chinese Herbal - administration & dosage
Drugs, Chinese Herbal - pharmacokinetics
Female
Humans
Jaundice - drug therapy
Jaundice - metabolism
Male
Rats
Rats, Wistar
Tablets - administration & dosage
动力学模型
大鼠
平板电脑
日常管理
时间间隔
药效学
高效液相色谱法
黄疸
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Summary:OBJECTIVE： To study the daily administration times of Canhuang tablet （CHT） for treating jaundice in rats based on a pharmacodynamic/pharma- cokinetic model. METHODS： Rats were modeled by 4% 1-Naphthylisothiocyanate （75 mg/kg, p.o.）. After 48 h, CHT was given （p.o.） at 0.75 g/kg once a day, 0.375 g/kg twice a day, and 0.25 g/kg three times a day. Blood was collected from the orbital sinus at different intervals. Levels of liver enzymes and bilirubin were detected using these blood samples. Bile was collected and determined after the first administration of CHT. High-performance liquid chromatography was used to determine the concentration of berberine in bile simultaneously. Time-effect and time-dose curves were then obtained.RESULTS： Compared with rats taking CHT twice and three times a day, the total amount of bile with- in 10 h of rats taking CHT once a day were 1.32- and 1.47-fold higher, respectively. There was good consistency between the pharmacokinetics of berberine and the pharmacodynamics of the effect on liver enzymes and bilirubin in vivo. The pharmacokinetic analyses showed that rats administered CHT once daily maintained a higher concentration of berberine in bile for a longer period than rats administered CHT twoand three-times daily. CONCLUSION： In jaundiced rats, taking CHT once a day is better than taking CHT twice or three times a day. These data may provide a reference for the clinical application of CHT.
ISSN:0255-2922