Comparison of visual and automated assessment of HER2 status and their impact on outcome in primary operable invasive ductal breast cancer

• Published in: Histopathology 2012-10, Vol.61 (4), p.675-684
• Main Authors: Mohammed, Zahra M A, Going, James J, McMillan, Donald C, Orange, Clare, Mallon, Elizabeth, Doughty, Julie C, Edwards, Joanne
• Format: Article
• Language: English
• Subjects: automated IHC method; Biological and medical sciences; Breast Neoplasms - metabolism; Breast Neoplasms - mortality; Breast Neoplasms - pathology; Carcinoma, Ductal, Breast - metabolism; Carcinoma, Ductal, Breast - mortality; Carcinoma, Ductal, Breast - pathology; Disease-Free Survival; Female; Gynecology. Andrology. Obstetrics; HER2 FISH test; Humans; Image Processing, Computer-Assisted - methods; Immunohistochemistry; In Situ Hybridization, Fluorescence; Investigative techniques, diagnostic techniques (general aspects); Mammary gland diseases; Medical sciences; Middle Aged; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; primary invasive breast cancer; Receptor, ErbB-2 - analysis; survival; Tissue Array Analysis; Tumors; visual IHC method
• ISSN: 0309-0167; 1365-2559
• DOI: 10.1111/j.1365-2559.2012.04280.x

Mohammed Z M A, Going J J, McMillan D C, Orange C, Mallon E, Doughty J C & Edwards J 
(2012) Histopathology 61, 675–684 Comparison of visual and automated assessment of HER2 status and their impact on outcome in primary operable invasive ductal breast cancer Aims:  To compare visual and computerized image analysis of HER2 immunohistochemistry (IHC) with fluorescence in‐situ hybridization (FISH) for HER2 status, and to examine the relationships with outcome in patients with primary operable invasive ductal breast cancer. Methods and results:  Tissue microarrays for 431 breast cancer patients were used to compare different approaches to the assessment of HER2 status. The cores were scored visually and with the Slidepath Tissue IA system, using the NICE‐approved scoring system for the HercepTest, as well as by FISH. The agreement between visual and image analysis of HER2 IHC was excellent [interclass correlation coefficient (ICCC) = 0.95, rs = 0.90, r = 0.91, k = 0.81, and P